0583675 - BC 2025 GB eng O - Others
Kachale, Ambar - Nenarokova, Anna - Roithová, Adriana - Durante, Ignacio Miguel - Miletínová, Petra - Záhonová, Kristýna - Nenarokov, Serafim - Votýpka, Jan - Horáková, Eva - Ross, R.L. - Yurchenko, V. - Beznosková, Petra - Paris, Zdeněk - Valášek, Leoš Shivaya - Lukeš, Julius … Total 16 authors
Short tRNA anticodon stem and mutant eRF1 allow stop codon reassignment (vol 613, pg 751, 2023).
2024. Nature. Palgrave Macmillan. Roč. 626, č. 7998 (2024), E4-E4. ISSN 0028-0836. E-ISSN 1476-4687
Institutional support: RVO:60077344 ; RVO:61388971
Keywords : tRNA * eRF1 * codon
OECD category: Biochemistry and molecular biology; Biochemistry and molecular biology (MBU-M)
Result website:
https://doi.org/10.1038/s41586-024-07065-0DOI: https://doi.org/10.1038/s41586-024-07065-0

After this article was published, we were alerted by Estienne Swart that the putative C. magnum 5-bp-long AS tRNATrp CCA, which we used as a negative control in Fig. 3d and 3f, most likely originated from a bacterial contaminant in the ciliate genome assembly. Using a metagenome binning strategy, we found that the contig containing the respective tRNA gene indeed belongs to a single bacterial metagenome-assembled genome (MAG) reconstructed from the whole-genome data. This high-quality MAG corresponds to an unidentified alphaproteobacterium not closely related to previously described taxa. We thus confirmed the bacterial origin of 5-bp AS tRNATrp. Regrettably, the presence of contaminating bacterial sequences in the C. magnum genome assembly was not obvious from the original paper1 when we were working on the original manuscript.
Permanent Link: https://hdl.handle.net/11104/0362221