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Effects of various environments on epigenetic settings and chromosomal damage
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SYSNO ASEP 0580305 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effects of various environments on epigenetic settings and chromosomal damage Author(s) Rössnerová, Andrea (UEM-P) RID
Elzeinová, Fatima (UEM-P)
Chvojková, Irena (UEM-P)
Hoňková, Kateřina (UEM-P)
Šíma, Michal (UEM-P) RID, ORCID
Milcová, Alena (UEM-P)
Pastorková, Anna (UEM-P) RID
Schmuczerová, Jana (UEM-P) RID
Rössner ml., Pavel (UEM-P) RID, ORCID
Topinka, Jan (UEM-P) RID, ORCID
Šrám, Radim (UEM-P) RIDArticle number 121290 Source Title Environmental Pollution. - : Elsevier - ISSN 0269-7491
Roč. 323, apr (2023)Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords air pollution ; chronical exposure ; epigenetic adaptation ; micronuclei ; chromosomal breaks ; NHEJ repair OECD category Public and environmental health R&D Projects EF16_019/0000798 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018133 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001821 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEM-P - RVO:68378041 UT WOS 000953151900001 EID SCOPUS 85148719802 DOI https://doi.org/10.1016/j.envpol.2023.121290 Annotation Air pollution is a dominant environmental exposure factor with significant health consequences. Unexpectedly, research in a heavily polluted region of the Czech Republic, with traditional heavy industry, revealed repeatedly the lowest frequency of micronuclei in the season with the highest concentrations of air pollutants including carcinogenic benzo[a]pyrene (B[a]P). Molecular findings have been collected for more than 10 years from various locations of the Czech Republic, with differing quality of ambient air. Preliminary conclusions have suggested adaptation of the population from the polluted locality (Ostrava, Moravian-Silesian Region (MSR)) to chronic air pollution exposure. In this study we utilize the previous findings and, for the first time, investigate micronuclei (MN) frequency by type: (i) centromere positive (CEN+) MN, representing chromosomal losses, and (ii) centromere negative (CEN-) MN representing chromosomal breaks. As previous results indicated differences between populations in the expression of XRCC5, a gene involved in the non-homologous end-joining (NHEJ) repair pathway, possible variations in epigenetic settings in this gene were also investigated. This new research was conducted in two seasons in the groups from two localities with different air quality levels (Ostrava (OS) and Prague (PG)). The obtained new results show significantly lower frequencies of chromosomal breaks in the OS subjects, related to the highest air pollution levels (p < 0.001). In contrast, chromosomal losses were comparable between both groups. In addition, significantly lower DNA methylation was found in 14.3% of the analyzed CpG loci of XRCC5 in the population from OS. In conclusion, the epigenetic adaptation (hypomethylation) in XRCC5 involved in the NHEJ repair pathway in the population from the polluted region, was suggested as a reason for the reduced level of chromosomal breaks. Further research is needed to explore the additional mechanisms, including genetic adaptation. Workplace Institute of Experimental Medicine Contact Arzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2024 Electronic address https://www.sciencedirect.com/science/article/pii/S0269749123002920?via%3Dihub
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