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Bacterial nanotubes as a manifestation of cell death
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SYSNO ASEP 0534616 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Bacterial nanotubes as a manifestation of cell death Author(s) Pospíšil, Jiří (MBU-M) ORCID, RID
Vítovská, Dragana (MBU-M) ORCID
Kofroňová, Olga (MBU-M) RID, ORCID
Muchová, K. (SK)
Šanderová, Hana (MBU-M) RID, ORCID
Hubálek, Martin (UOCHB-X) RID, ORCID
Šiková, Michaela (MBU-M) ORCID
Modrák, Martin (MBU-M) ORCID
Benada, Oldřich (MBU-M) ORCID, RID
Barák, I. (SK)
Krásný, Libor (MBU-M) RID, ORCIDArticle number 4963 Source Title Nature Communications. - : Nature Publishing Group - ISSN 2041-1723
Roč. 11, č. 1 (2020)Number of pages 12 s. Language eng - English Country GB - United Kingdom Keywords outer-membrane vesicles ; bacillus-subtilis ; peptidoglycan ; competence ; expression ; flagella ; reveals Subject RIV EE - Microbiology, Virology OECD category Microbiology Subject RIV - cooperation Institute of Organic Chemistry and Biochemistry - Analytical Chemistry, Separation R&D Projects GA19-12956S GA ČR - Czech Science Foundation (CSF) LO1509 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018131 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) NV17-29680A GA MZd - Ministry of Health (MZ) Research Infrastructure ELIXIR-CZ - 90047 - Ústav organické chemie a biochemie AV ČR, v. v. i. Method of publishing Open access Institutional support MBU-M - RVO:61388971 ; UOCHB-X - RVO:61388963 UT WOS 000577122000001 EID SCOPUS 85091873399 DOI https://doi.org/10.1038/s41467-020-18800-2 Annotation Bacterial nanotubes are membranous structures that have been reported to function as conduits between cells to exchange DNA, proteins, and nutrients. Here, we investigate the morphology and formation of bacterial nanotubes using Bacillus subtilis. We show that nanotube formation is associated with stress conditions, and is highly sensitive to the cells' genetic background, growth phase, and sample preparation methods. Remarkably, nanotubes appear to be extruded exclusively from dying cells, likely as a result of biophysical forces. Their emergence is extremely fast, occurring within seconds by cannibalizing the cell membrane. Subsequent experiments reveal that cell-to-cell transfer of non-conjugative plasmids depends strictly on the competence system of the cell, and not on nanotube formation. Our study thus supports the notion that bacterial nanotubes are a post mortem phenomenon involved in cell disintegration, and are unlikely to be involved in cytoplasmic content exchange between live cells. Bacterial nanotubes and other similar membranous structures have been reported to function as conduits between cells to exchange DNA, proteins, and nutrients. Here the authors provide evidence that bacterial nanotubes are formed only by dead or dying cells, thus questioning their previously proposed functions. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://www.nature.com/articles/s41467-020-18800-2
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