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The role of chloroplasts in the oxidative stress that is induced by zearalenone in wheat plants - The functions of 24-epibrassinolide and selenium in the protective mechanisms
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SYSNO ASEP 0503951 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The role of chloroplasts in the oxidative stress that is induced by zearalenone in wheat plants - The functions of 24-epibrassinolide and selenium in the protective mechanisms Author(s) Filek, M. (PL)
Sieprawska, A. (PL)
Kościelniak, J. (PL)
Oklešťková, Jana (UEB-Q) RID, ORCID, SAI
Jurczyk, B. (PL)
Telk, A. (PL)
Biesaga-Kościelniak, J. (PL)
Janeczko, A. (PL)Number of authors 8 Source Title Plant Physiology and Biochemistry. - : Elsevier - ISSN 0981-9428
Roč. 137, APR (2019), s. 84-92Number of pages 9 s. Language eng - English Country FR - France Keywords Brassinosteroids ; Chloroplasts ; Hsp90 ; Photosynthesis ; Selenium ; Wheat ; Zearalenone Subject RIV EF - Botanics OECD category Plant sciences, botany Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000462106100008 EID SCOPUS 85061324819 DOI 10.1016/j.plaphy.2019.01.030 Annotation This study focused on the idea that the toxic effect of zearalenone (ZEA) and the protective actions of the brassinosteroid 24-epibrassinolide (EBR) as well as selenium are dependent on its accumulation in chloroplasts to a high degree. These organelles were isolated from the leaves of oxidative stress-sensitive and stress-tolerant wheat cultivars that had been grown from grains that had been incubated in a solution of ZEA (30 μM), Na 2 SeO 4 (Se, 10 μM), EBR (0.1 μM) or in a mixture of ZEA with Se or EBR. Ultra-high performance liquid chromatography techniques indicated that ZEA was adsorbed in higher amounts in the chloroplasts in the sensitive rather than tolerant cultivar. Although the brassinosteroids and Se were also accumulated in the chloroplasts, higher levels were only found in the tolerant cultivar. The application of EBR increased the homocastasterone content, especially in the chloroplasts of the tolerant plant and after the addition of ZEA. The presence of both protectants caused a decrease in the ZEA content in studied organelles and resulted in diminishing of the oxidative stress (i.e. changes in the activity of the antioxidative enzymes). Moreover, a recovery of photosystem II and decrease in the negative impact of ZEN on Hsp90 transcript accumulation was observed in plants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020 Electronic address http://doi.org/10.1016/j.plaphy.2019.01.030
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