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Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness
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SYSNO ASEP 0488777 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness Author(s) Minina, E. A. (SE)
Moschou, P. N. (SE)
Vetukuri, R. R. (SE)
Sanchez-Vera, V. (SE)
Cardoso, C. (SE)
Liu, Q. (CN)
Elander, P. H. (SE)
Dalman, K. (SE)
Beganovic, M. (SE)
Lindberg Yilmaz, J. (SE)
Marmon, S. (SE)
Shabala, L. (AU)
Suarez, M. (ES)
Ljung, K. (SE)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Shabala, S. (AU)
Stymne, S. (SE)
Hofius, D. (SE)
Bozhkov, P. V. (SE)Number of authors 19 Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 69, č. 6 (2018), s. 1415-1432Number of pages 18 s. Language eng - English Country GB - United Kingdom Keywords Aging ; ATG genes ; Autophagy ; Autophagy-related ubiquitin-like conjugation systems ; Biomass ; Oil content ; Ratelimiting components of autophagic flux ; Seed yield ; Stress resistance ; Transcriptional regulation Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) Institutional support UEB-Q - RVO:61389030 UT WOS 000427685700011 EID SCOPUS 85044200714 DOI 10.1093/jxb/ery010 Annotation Autophagy is a major catabolic process whereby autophagosomes deliver cytoplasmic content to the lytic compartment for recycling. Autophagosome formation requires two ubiquitin-like systems conjugating Atg12 with Atg5, and Atg8 with lipid phosphatidylethanolamine (PE), respectively. Genetic suppression of these systems causes autophagy-deficient phenotypes with reduced fitness and longevity. We show that Atg5 and the E1-like enzyme, Atg7, are rate-limiting components of Atg8PE conjugation in Arabidopsis. Overexpression of ATG5 or ATG7 stimulates Atg8 lipidation, autophagosome formation, and autophagic flux. It also induces transcriptional changes opposite to those observed in atg5 and atg7 mutants, favoring stress resistance and growth. As a result, ATG5- or ATG7-overexpressing plants exhibit increased resistance to necrotrophic pathogens and oxidative stress, delayed aging and enhanced growth, seed set, and seed oil content. This work provides an experimental paradigm and mechanistic insight into genetic stimulation of autophagy in planta and shows its efficiency for improving plant productivity. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2019
Number of the records: 1