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Metabolism of ibuprofen in higher plants: A model Arabidopsis thaliana cell suspension culture system
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SYSNO ASEP 0481319 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Metabolism of ibuprofen in higher plants: A model Arabidopsis thaliana cell suspension culture system Author(s) Maršík, Petr (UEB-Q) RID, ORCID
Šíša, Miroslav (UEB-Q) ORCID
Lacina, O. (CZ)
Moťková, Kateřina (UEB-Q) ORCID
Langhansová, Lenka (UEB-Q) RID, ORCID
Rezek, Jan (UEB-Q) RID, ORCID
Vaněk, Tomáš (UEB-Q) RID, ORCIDNumber of authors 7 Source Title Environmental Pollution. - : Elsevier - ISSN 0269-7491
Roč. 220, JAN (2017), s. 383-392Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords Arabidopsis thaliana ; Ibuprofen ; Metabolism ; Plant cells ; Sequestration Subject RIV CE - Biochemistry OECD category Plant sciences, botany R&D Projects GA14-22593S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000390736700041 EID SCOPUS 84992163553 DOI 10.1016/j.envpol.2016.09.074 Annotation The uptake and metabolism of ibuprofen (IBU) by plants at the cellular level was investigated using a suspension culture of A. thaliana. Almost all IBU added to the medium (200 μM) was metabolized or bound to insoluble structures in 5 days. More than 300 metabolites were determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis, and most of these are first reported for plants here. Although hydroxylated derivatives formed by oxidation on the isobutyl side chain were the main first-step products of IBU degradation, conjugates of these products with sugar, methyl and amino acid groups were the dominant metabolites in the culture. The main portion of total added IBU (81%) was accumulated in the extractable intracellular pool, whereas the cultivation medium fraction contained only 19%. The amount of the insoluble cell-wall-bound IBU was negligible (0.005% of total IBU). Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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