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An Automated Method for High-Throughput Screening of Arabidopsis Rosette Growth in Multi-Well Plates and Its Validation in Stress Conditions
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SYSNO ASEP 0480124 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title An Automated Method for High-Throughput Screening of Arabidopsis Rosette Growth in Multi-Well Plates and Its Validation in Stress Conditions Author(s) De Diego, N. (CZ)
Fürst, T. (CZ)
Humplík, Jan (UEB-Q) ORCID
Ugena, L. (CZ)
Podlešáková, K. (CZ)
Spíchal, L. (CZ)Number of authors 6 Article number 1702 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 8, OCT 4 (2017)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords salt stress ; chlorophyll fluorescence ; salinity tolerance ; plant-responses ; cold-tolerance ; water-deficit ; thaliana ; selection ; platform ; reveals ; high-throughput screening assay ; Arabidopsis ; multi-well plates ; rosette growth ; stress conditions Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000412220500001 DOI 10.3389/fpls.2017.01702 Annotation High-throughput plant phenotyping platforms provide new possibilities for automated, fast scoring of several plant growth and development traits, followed over time using non-invasive sensors. Using Arabidopsis as a model offers important advantages for high-throughput screening with the opportunity to extrapolate the results obtained to other crops of commercial interest. In this study we describe the development of a highly reproducible high-throughput Arabidopsis in vitro bioassay established using our OloPhen platform, suitable for analysis of rosette growth in multi-well plates. This method was successfully validated on example of multivariate analysis of Arabidopsis rosette growth in different salt concentrations and the interaction with varying nutritional composition of the growth medium. Several traits such as changes in the rosette area, relative growth rate, survival rate and homogeneity of the population are scored using fully automated RGB imaging and subsequent image analysis. The assay can be used for fast screening of the biological activity of chemical libraries, phenotypes of transgenic or recombinant inbred lines, or to search for potential quantitative trait loci. It is especially valuable for selecting genotypes or growth conditions that improve plant stress tolerance. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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