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Cytogenetic relationships within the Maghrebian clade of Festuca subgen. Schedonorus (Poaceae), using flow cytometry and FISH
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SYSNO ASEP 0478868 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cytogenetic relationships within the Maghrebian clade of Festuca subgen. Schedonorus (Poaceae), using flow cytometry and FISH Author(s) Ezquerro-López, D. (ES)
Kopecký, David (UEB-Q) RID, ORCID
Inda, L. A. (ES)Number of authors 3 Article number e052 Source Title Jardin Botanico de Madrid. Anales . - : Consejo Superior de Investigaciones Cientificas (C S I C) * Departamento de Publicaciones - ISSN 0211-1322
Roč. 74, č. 1 (2017)Number of pages 9 s. Language eng - English Country ES - Spain Keywords Broad-leaved fescues ; Festuca subgen. Schedonorus ; fish ; Genome size ; rdna Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany Institutional support UEB-Q - RVO:61389030 UT WOS 000408455300006 EID SCOPUS 85023188672 DOI 10.3989/ajbm.2455 Annotation Festuca subgen. Schedonorus is a group of broad-leaved fescues, which can be divided into two clades: European and Maghrebian. We employed fluorescent in situ hybridization -FISH- with probes specific for 5S and 35S ribosomal DNA and genome size estimation using flow cytometry to shed light on the determination of possible parental genomes of polyploid species of the Maghrebian clade. Our results indicate that octoploid F. arundinacea subsp. atlantigena probably originated from crossing ofthe tetraploids F. arundinacea subsp. fenas -2n = 4x = 28- and F. mairei -2n = 4x = 28- followed by whole genome duplication. However, a large reconstruction of karyotype and genome downsizing has been revealed. Similarly, hexaploid F. arundinacea subsp. corsica presumably resulted from the interspecific hybridization of the diploid F. pratensis and tetraploid F. arundinacea subsp. fenas. Several scenarios on the origin of decaploid F. arundinacea var. letourneuxiana are discussed. This study contributed to our knowledge on the phylogeny of broad-leavedfescues and provided new information on the karyotypes - chromosomenumbers, ploidy levels and numbers and positions of rDNA loci-using FISH and genome size estimations using flow cytometry in selected taxa of this important grass genus. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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