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Membrane Binding of Recoverin: From Mechanistic Understanding to Biological Functionality
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SYSNO ASEP 0478753 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Membrane Binding of Recoverin: From Mechanistic Understanding to Biological Functionality Author(s) Timr, S. (CZ)
Pleskot, Roman (UEB-Q) RID, ORCID
Kadlec, J. (CZ)
Kohagen, M. (CZ)
Magarkar, A. (CZ)
Jungwirth, P. (CZ)Number of authors 6 Source Title ACS Central Science. - : American Chemical Society - ISSN 2374-7943
Roč. 3, č. 8 (2017), s. 868-874Number of pages 7 s. Language eng - English Country US - United States Keywords recoverin ; membrane ; myristoyl Subject RIV CF - Physical ; Theoretical Chemistry OECD category Cell biology Institutional support UEB-Q - RVO:61389030 UT WOS 000408141900011 EID SCOPUS 85028063042 DOI 10.1021/acscentsci.7b00210 Annotation Recoverin is a neuronal calcium sensor involved in vision adaptation that reversibly associates with cellular membranes via its calcium-activated myristoyl switch. While experimental evidence shows that the myristoyl group significantly enhances membrane affinity of this protein, molecular details of the binding process are still under debate. Here, we present results of extensive molecular dynamics simulations of recoverin in the proximity of a phospholipid bilayer. We capture multiple events of spontaneous membrane insertion of the myristoyl moiety and confirm its critical rol e in the membrane binding. Moreover, we observe that the binding strongly depends on the conformation of the N-terminal domain. We propose that a suitable conformation of the N-terminal domain can be stabilized by the disordered C-terminal segment or by binding of the target enzyme, i.e., rhodopsin kinase. Finally, we find that the presence of negatively charged lipids in the bilayer stabilizes a physiologically functional orientation of the membrane-bound recoverin. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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