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Brassinosteroid biosynthesis is modulated via a transcription factor cascade of COG1, PIF4, and PIF5
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SYSNO ASEP 0476568 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Brassinosteroid biosynthesis is modulated via a transcription factor cascade of COG1, PIF4, and PIF5 Author(s) Wei, Z. (CN)
Yuan, T. (US)
Tarkowská, Danuše (UEB-Q) RID, ORCID
Kim, J. (KR)
Nam, H. G. (KR)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
He, K. (CN)
Gou, X. (CN)
Li, J. (CN)Number of authors 9 Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 174, č. 2 (2017), s. 1260-1273Number of pages 14 s. Language eng - English Country US - United States Keywords GSK3-LIKE KINASE BIN2 ; ARABIDOPSIS-THALIANA ; CATHARANTHUS-ROSEUS Subject RIV EF - Botanics OECD category Plant sciences, botany R&D Projects LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA14-34792S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000403152200064 EID SCOPUS 85020482386 DOI https://doi.org/10.1104/pp.16.01778 Annotation Brassinosteroids (BRs) are essential phytohormones regulating various developmental and physiological processes during normal growth and development. cog1-3D (cogwheel1-3D) was identified as an activation-tagged genetic modifier of bri1-5, an intermediate BR receptor mutant in Arabidopsis (Arabidopsis thaliana). COG1 encodes a Dof-type transcription factor found previously to act as a negative regulator of the phytochrome signaling pathway. cog1-3D single mutants show an elongated hypocotyl phenotype under light conditions. A loss-of-function mutant or inducible expression of a dominant negative form of COG1 in the wild type results in an opposite phenotype. A BR profile assay indicated that BR levels are elevated in cog1-3D seedlings. Quantitative reverse transcription-polymerase chain reaction analyses showed that several key BR biosynthetic genes are significantly up-regulated in cog1-3D compared with those of the wild type. Two basic helix-loop-helix transcription factors, PIF4 and PIF5, were found to be transcriptionally up-regulated in cog1-3D. Genetic analysis indicated that PIF4 and PIF5 were required for COG1 to promote BR biosynthesis and hypocotyl elongation. Chromatin immunoprecipitation and electrophoretic mobility shift assays indicated that COG1 binds to the promoter regions of PIF4 and PIF5, and PIF4 and PIF5 bind to the promoter regions of key BR biosynthetic genes, such as DWF4 and BR6ox2, to directly promote their expression. These results demonstrated that COG1 regulates BR biosynthesis via up-regulating the transcription of PIF4 and PIF5. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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