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In vitro antiinflammatory and antioxidant potential of root extracts from Ranunculaceae species
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SYSNO ASEP 0476506 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title In vitro antiinflammatory and antioxidant potential of root extracts from Ranunculaceae species Author(s) Malík, J. (CZ)
Tauchen, J. (CZ)
Landa, Přemysl (UEB-Q) RID, ORCID
Kutil, Zsófia (UEB-Q)
Maršík, Petr (UEB-Q) RID, ORCID
Kloucek, P. (CZ)
Havlík, J. (CZ)
Kokoška, L. (CZ)Number of authors 8 Source Title South African Journal of Botany. - : Elsevier - ISSN 0254-6299
Roč. 109, MAR (2017), s. 128-137Number of pages 10 s. Language eng - English Country ZA - South Africa Keywords Buttercup family ; Cyclooxygenase, fatty acids ; Lipoxygenase, medicinal plants ; Plant extracts Subject RIV GM - Food Processing OECD category Plant sciences, botany R&D Projects GA16-07193S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000400465400016 EID SCOPUS 85009121766 DOI 10.1016/j.sajb.2016.12.008 Annotation Among the ethanol extracts of roots of 25 Ranunculaceous plant species traditionally used in treatment of various inflammatory diseases, Helleborus purpurascens demonstrated the most promising inhibitory activity towards COX-1, COX-2 and 5-LOX enzymes (IC 50 detected as 5.64, 10.33 and 3.84 μg/mL, respectively). GC–MS analysis of subfractions obtained from H. purpurascens extract revealed that a complex of fatty acids (especially α-linolenic acid) might be responsible for the observed antiinflammatory activity. The extract of Thalictrum minus was found to be selectively inhibiting COX-2 and 5-LOX enzymes (IC 50 = 49.68 and 76.47 μg/mL, respectively) but had no inhibitory effect on COX-1 (IC 50 > 128 μg/mL). Moreover, T. minus extract also showed significant antioxidant effect (DPPH = 0.074 g TE/g, and ORAC = 0.518 g TE/g) as well as was relatively rich on phenolic compounds (TPC = 0.352 g GAE/g). Statistical analysis showed that phenolic compounds might be only partially responsible for antioxidant potential demonstrated by this plant. The results of our study suggest that the above-mentioned members of Ranunculaceae family may possibly serve as a prospective material for further development of novel and safer plant-based antiinflammatory and/or antioxidant agents. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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