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Genetic Diversity of Blumeria graminis f. sp hordei in Central Europe and Its Comparison with Australian Population
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SYSNO ASEP 0467790 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Genetic Diversity of Blumeria graminis f. sp hordei in Central Europe and Its Comparison with Australian Population Author(s) Komínková, Eva (UEB-Q) RID, ORCID
Dreiseitl, A. (CZ)
Malečková, Eva (UEB-Q)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Valárik, Miroslav (UEB-Q) RID, ORCIDNumber of authors 5 Article number e0167099 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 11, č. 11 (2016)Number of pages 15 s. Language eng - English Country US - United States Keywords barley powdery mildew ; f.sp hordei ; isozyme variation ; ssr markers ; virulence ; pathogen ; complexity ; france ; frequencies ; resistance Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LD14105 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000388886000049 DOI 10.1371/journal.pone.0167099 Annotation Population surveys of Blumeria graminis f. sp. hordei (Bgh), a causal agent of more than 50% of barley fungal infections in the Czech Republic, have been traditionally based on virulence tests, at times supplemented with non-specific Restriction fragment length polymorphism or Random amplified polymorphic DNA markers. A genomic sequence of Bgh, which has become available recently, enables identification of potential markers suitable for population genetics studies. Two major strategies relying on transposable elements and micro satellites were employed in this work to develop a set of Repeat junction markers, Single sequence repeat and Single nucleotide polymorphism markers. A resolution power of the new panel of markers comprising 33 polymorphisms was demonstrated by a phylogenetic analysis of 158 Bgh isolates. A core set of 97 Czech isolates was compared to a set 50 Australian isolates on the background of 11 diverse isolates collected throughout the world. 73.2% of Czech isolates were found to be genetically unique. An extreme diversity of this collection was in strong contrast with the uniformity of the Australian one. This work paves the way for studies of population structure and dynamics based on genetic variability among different Bgh isolates originating from geographically limited regions. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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