Number of the records: 1
Synthesis of 3 alpha-deuterated 7 alpha-hydroxy-DHEA and 7-oxo-DHEA and application in LC-MS/MS plasma analysis
- 1.
SYSNO ASEP 0463516 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Synthesis of 3 alpha-deuterated 7 alpha-hydroxy-DHEA and 7-oxo-DHEA and application in LC-MS/MS plasma analysis Author(s) Sosvorova, L.K. (CZ)
Sarek, J. (CZ)
Vitku, J. (CZ)
Kvasnica, Miroslav (UEB-Q) RID, ORCIDSource Title Steroids. - : Elsevier - ISSN 0039-128X
Roč. 112, AUG (2016), s. 88-94Number of pages 7 s. Language eng - English Country US - United States Keywords 7 alpha-Hydroxy-DHEA ; 7-Oxo-DHEA ; Deuterated standard Subject RIV CE - Biochemistry Institutional support UEB-Q - RVO:61389030 UT WOS 000380602100011 DOI https://doi.org/10.1016/j.steroids.2016.05.001 Annotation 7-Oxygenated metabolites of dehydroepiandrosterone (DHEA) are known for their neuroprotective and immunomodulatory properties. These neuroactive steroids are currently predominately analysed by mass spectrometry, for which the use of internal deuterated standards is necessary. The aim of this study was to synthesize the deuterated derivatives of 7 alpha-hydroxy-DHEA and 7-oxo-DHEA and test them in liquid chromatography-tandem mass spectrometry (LC-MS/MS) in order to enhance the performance characteristics of this method. Here we report the synthesis of 3 alpha deuterium-labelled 7 alpha-hydroxy-DHEA and 7-oxo-DHEA. Deuterium was introduced into the 3 alpha position by reduction of the corresponding 3-ketone with a protected 17-carbonyl group using NaBD4. Our new procedure allows the easier synthesis of deuterated steroid labelled compounds. The use of these deuterated steroids enabled us to improve the human plasma LC-MS/MS analysis of 7 alpha-hydroxy-DHEA and 7-oxo-DHEA in terms of sensitivity, precision and recovery. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
Number of the records: 1