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Leukotrienes in Exhaled Breath Condensate and Fractional Exhaled Nitric Oxide in Workers Exposed to TiO2 Nanoparticles.
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SYSNO ASEP 0460510 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Leukotrienes in Exhaled Breath Condensate and Fractional Exhaled Nitric Oxide in Workers Exposed to TiO2 Nanoparticles. Author(s) Pelclová, D. (CZ)
Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
Kačer, P. (CZ)
Felclová, Z. (CZ)
Vlčková, Š. (CZ)
Komarc, M. (CZ)
Navrátil, Tomáš (UFCH-W) RID, ORCID
Schwarz, Jaroslav (UCHP-M) RID, ORCID, SAI
Zíková, Naděžda (UCHP-M) RID, ORCID, SAI
Makeš, Otakar (UCHP-M) RID, ORCID, SAI
Syslová, K. (CZ)
Běláček, J. (CZ)
Zakharov, S. (CZ)Source Title Journal of Breath Research. - : Institute of Physics Publishing - ISSN 1752-7155
Roč. 10, č. 3 (2016), s. 036004Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords nanoparticles ; TiO2 ; exhaled breath condensate Subject RIV CF - Physical ; Theoretical Chemistry Subject RIV - cooperation J. Heyrovsky Institute of Physical Chemistry - Electrochemistry Institutional support UCHP-M - RVO:67985858 ; UFCH-W - RVO:61388955 UT WOS 000378999600006 EID SCOPUS 85002044896 DOI 10.1088/1752-7155/10/3/036004 Annotation Human health data regarding exposure to nanoparticles are extremely scarce and biomonitoring of exposure is lacking in spite of rodent pathological experimental data. Potential markers of the
health-effects of engineered nanoparticles were examined in 30 workers exposed to TiO2 aerosol, 22 office employees of the same plant, and 45 unexposed controls. Leukotrienes (LT) B4, C4, E4, and D4 were analysed in the exhaled breath condensate (EBC) and urine via liquid chromatography– electrospray ionization–tandem mass spectrometry (LC-ESI-MS/MS). Fractional exhaled nitric
oxide (FeNO) and spirometry was also measured. The median particle number concentration of the aerosol in the production ranged from 1.98 × 104 to 2.32 × 104 particles cm−3; about 80% of
the particles were <100 nm in diameter. Median total mass concentration varied between 0.4 and 0.65 mg m−3. All LT levels in workers’ EBC were elevated relative to the controls (p < 0.01). LTs in
the EBC sample were correlated with titanium levels. Urinary LTs were not elevated in the workers and office employees. Office workers had higher LTB4 in EBC (p < 0.05), and higher levels of FeNO
(p < 0.01). FeNO was higher in office employees with allergic diseases and was negatively correlated with smoking (p < 0.01). In spirometry significant impairment in the workers was seen only for
%VCIN and %PEF (both p < 0.01). Multiple regression analysis confirmed a significant association between production of TiO2 and all cysteinyl LTs in EBC (p < 0.01) and impaired %VCIN and %PEF
(both p < 0.01). LTB4 was also associated with smoking (p < 0.01). LT levels complemented our earlier findings of DNA, protein, and lipid damage in the EBC of workers with nanoTiO2 exposures.
Cysteinyl LTs in EBC analysis suggest inflammation and potential fibrotic changes in the lungs; they may be helpful for monitoring the biological effect of (nano)TiO2 on workers. Spirometry was not
sensitive enough.Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2017
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