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Hormone-mediated growth dynamics of the barley pericarp as revealed by magnetic resonance imaging and transcript profiling
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SYSNO ASEP 0452595 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Hormone-mediated growth dynamics of the barley pericarp as revealed by magnetic resonance imaging and transcript profiling Author(s) Pielot, R. (DE)
Kohl, S. (DE)
Manz, B. (DE)
Rutten, T. (DE)
Weier, D. (DE)
Tarkowská, Danuše (UEB-Q) RID, ORCID
Rolčík, Jakub (UEB-Q)
Strnad, Miroslav (UEB-Q) RID, ORCID
Volke, F. (DE)
Weber, H. (DE)
Weschke, W. (DE)Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 66, č. 21 (2015), s. 6927-6943Number of pages 17 s. Language eng - English Country GB - United Kingdom Keywords Auxin ; barley pericarp ; cell expansion Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA14-34792S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000367814500030 DOI 10.1093/jxb/erv397 Annotation The shape of the maternal pericarp affects cereal grain mass and yield. Pericarp growth was analysed by magnetic resonance imaging (MRI), revealing topological maps of mobile water in developing pericarp of barley (Hordeum vulgare) and displaying tissue regions actively elongating in specific temporal-spatial patterns. Correlation analysis of MRI signals and growth rates reveals that growth in length is mediated by dorsal and also lateral rather than ventral regions. Growth in thickness is related to ventral regions. Switching from dorsal to ventral growth is associated with differential expression of axial regulators of the HD-ZipIII and Kanadi/Ettin types, and NPH3 photoreceptors, suggesting light-mediated auxin re-distribution. Auxin increases with the highest levels in the basal pericarp at 6 days after fertilization (DAF), together with transcriptionally up-regulated auxin transport and signalling. Gibberellin biosynthesis is transcriptionally up-regulated only later, and levels of bioactive gibberellins increase from 7 to 13 DAF, with higher levels in ventral than dorsal regions. Differential gene expression related to cell expansion indicates genes related to apoplast acidification, wall relaxation, sugar cleavage, water transport, and cell wall biosynthesis. Candidate genes potentially involved in pericarp extension are distinguished by their temporal expression, representing potential isoforms responsible for dorsal-mediated early growth in length or ventral-mediated late growth in thickness. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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