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Measuring Meiotic Crossovers via Multi-Locus Genotyping of Single Pollen Grains in Barley
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SYSNO ASEP 0448069 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Measuring Meiotic Crossovers via Multi-Locus Genotyping of Single Pollen Grains in Barley Author(s) Dreissig, S. (DE)
Fuchs, J. (DE)
Cápal, Petr (UEB-Q) RID, ORCID
Kettles, N. (GB)
Byrne, E. (GB)
Houben, A. (GB)Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 10, č. 9 (2015), e0137677Number of pages 10 s. Language eng - English Country US - United States Keywords RECOMBINATION LANDSCAPE ; ARABIDOPSIS ; PLANTS Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000360965800077 DOI 10.1371/journal.pone.0137677 Annotation The detection of meiotic crossovers in crop plants currently relies on scoring DNA markers in a segregating population or cytological visualization. We investigated the feasibility of using flow-sorted haploid nuclei, Phi29 DNA polymerase-based whole-genome-amplification (WGA) and multi-locus KASP-genotyping to measure meiotic crossovers in individual barley pollen grains. To demonstrate the proof of concept, we used 24 gene-based physically mapped single nucleotide polymorphisms to genotype the WGA products of 50 single pollen nuclei. The number of crossovers per chromosome, recombination frequencies along chromosome 3H and segregation distortion were analysed and compared to a doubled haploid (DH) population of the same genotype. The number of crossovers and chromosome wide recombination frequencies show that this approach is able to produce results that resemble those obtained from other methods in a biologically meaningful way. Only the segregation distortion was found to be lower in the pollen population than in DH plants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
Number of the records: 1