Number of the records: 1
A high-resolution physical map integrating an anchored chromosome with the BAC physical maps of wheat chromosome 6B
- 1.
SYSNO ASEP 0447326 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A high-resolution physical map integrating an anchored chromosome with the BAC physical maps of wheat chromosome 6B Author(s) Kobayashi, F. (JP)
Wu, J.Z. (JP)
Kanamori, H. (JP)
Tanaka, T. (JP)
Katagiri, S. (JP)
Karasawa, W. (JP)
Kaneko, S. (JP)
Watanabe, S. (JP)
Sakaguchi, T. (JP)
Šafář, Jan (UEB-Q) RID, ORCID
Šimková, Hana (UEB-Q) RID, ORCID
Mukai, Y. (JP)
Hamada, M. (JP)
Saito, M. (JP)
Hayakawa, K. (JP)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Nasuda, S. (JP)
Matsumoto, T. (JP)
Handa, H. (JP)Number of authors 32 Source Title BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 16, AUG 12 (2015), s. 595Number of pages 21 s. Language eng - English Country US - United States Keywords Centromere ; Chromosomal rearrangement ; Chromosome 6B Subject RIV EB - Genetics ; Molecular Biology R&D Projects GBP501/12/G090 GA ČR - Czech Science Foundation (CSF) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000359344000002 DOI 10.1186/s12864-015-1803-y Annotation Background: A complete genome sequence is an essential tool for the genetic improvement of wheat. Because the wheat genome is large, highly repetitive and complex due to its allohexaploid nature, the International Wheat Genome Sequencing Consortium (IWGSC) chose a strategy that involves constructing bacterial artificial chromosome (BAC)-based physical maps of individual chromosomes and performing BAC-by-BAC sequencing. Here, we report the construction of a physical map of chromosome 6B with the goal of revealing the structural features of the third largest chromosome in wheat. Results: We assembled 689 informative BAC contigs (hereafter reffered to as contigs) representing 91 % of the entire physical length of wheat chromosome 6B. The contigs were integrated into a radiation hybrid (RH) map of chromosome 6B, with one linkage group consisting of 448 loci with 653 markers. The order and direction of 480 contigs, corresponding to 87 % of the total length of 6B, were determined. We also characterized the contigs that contained a part of the nucleolus organizer region or centromere based on their positions on the RH map and the assembled BAC clone sequences. Analysis of the virtual gene order along 6B using the information collected for the integrated map revealed the presence of several chromosomal rearrangements, indicating evolutionary events that occurred on chromosome 6B. Conclusions: We constructed a reliable physical map of chromosome 6B, enabling us to analyze its genomic structure and evolutionary progression. More importantly, the physical map should provide a high-quality and map-based reference sequence that will serve as a resource for wheat chromosome 6B. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
Number of the records: 1