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Mutagenesis during plant responses to UVB radiation
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SYSNO ASEP 0447257 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mutagenesis during plant responses to UVB radiation Author(s) Holá, Marcela (UEB-Q) ORCID
Vágnerová, Radka (UEB-Q) ORCID
Angelis, Karel (UEB-Q) RID, ORCIDSource Title Plant Physiology and Biochemistry. - : Elsevier - ISSN 0981-9428
Roč. 93, SI (2015), s. 29-33Number of pages 5 s. Language eng - English Country FR - France Keywords UV dimers ; DNA repair ; Error-prone bypass Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA13-06595S GA ČR - Czech Science Foundation (CSF) LD13006 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000357223100005 DOI 10.1016/j.plaphy.2014.12.013 Annotation We tested an idea that induced mutagenesis due to unrepaired DNA lesions, here the UV photoproducts, underlies the impact of UVB irradiation on plant phenotype. For this purpose we used protonemal culture of the moss Physcomitrella patens with 50% of apical cells, which mimics actively growing tissue, the most vulnerable stage for the induction of mutations. We measured the UVB mutation rate of various moss lines with defects in DNA repair (pplig4, ppku70, pprad50, ppmre11), and in selected clones resistant to 2-Fluoroadenine, which were mutated in the adenosine phosphotrasferase gene (APT), we analysed induced mutations by sequencing. In parallel we followed DNA break repair and removal of cyclobutane pyrimidine dimers with a half-life tau = 4 h 14 min determined by comet assay combined with UV dimer specific T4 endonuclease V. We show that UVB induces massive, sequence specific, error-prone bypass repair that is responsible for a high mutation rate owing to relatively slow, though error-free, removal of photoproducts by nucleotide excision repair (NER). Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
Number of the records: 1