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Tobacco susceptibility to Potato virus Y-NTN infection is affected by grafting and endogenous cytokinin content
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SYSNO ASEP 0446882 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Tobacco susceptibility to Potato virus Y-NTN infection is affected by grafting and endogenous cytokinin content Author(s) Spoustová, Petra (UEB-Q)
Hýsková, V. (CZ)
Müller, Karel (UEB-Q) RID, ORCID
Schnablová, Renáta (UEB-Q) RID, ORCID
Ryšlavá, H. (CZ)
Čeřovská, Noemi (UEB-Q) RID, ORCID
Malbeck, Jiří (UEB-Q) RID, ORCID
Cvikrová, Milena (UEB-Q) RID, ORCID
Synková, Helena (UEB-Q) RID, ORCIDSource Title Plant Science. - : Elsevier - ISSN 0168-9452
Roč. 235, JUN 2015 (2015), s. 25-36Number of pages 12 s. Language eng - English Country IE - Ireland Keywords Cytokinin ; ipt gene ; Antioxidant system Subject RIV EF - Botanics Institutional support UEB-Q - RVO:61389030 UT WOS 000354591700003 DOI 10.1016/j.plantsci.2015.02.017 Annotation Faster or stronger response to pathogen occurs if plants undergo prior priming. Cytokinins seem to be also involved in plant priming and in response to pathogens. Susceptibility to Potato virus Y-NTN RVYNTN was studied in transgenic cytokinin overproducing (Pssu-ipt) tobacco and compared with nontransgenic plants. Since cytokinin overproduction inhibits development of plant roots and grafting overcomes this limitation, both types were grown as rooted and/or grafted plants to check also the effect of grafting. Control rooted tobacco (C), the most susceptible to PVYNTN, showed always symptoms during the infection together with the rising virus content and a systemic response, such as accumulation of H2O2, salicylic acid (SA) and other phenolic acids, and stress-induced enzyme activities. In transgenic and grafted plants, the response to PVYNTN was dependent on protective mechanisms activated prior to the inoculation. In Pssu-ipt tobacco, cytokinin active forms and SA contents exceeded manifold their content in C. Grafting promoted the accumulation of phenolics, but SA, and stimulated peroxidase activities. Thus, the pre-infection barrier established in both transgenic and grafted plants helped to suppress partly the virus multiplication and resulted in milder symptom development. However, only the synergic effect of both grafting and the high cytokinins led to PVYNTN tolerance in transgenic grafts. Possible mechanisms were discussed. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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