The influence of the quinone antioxidants tert-butylhydroquinone and 2,5-di-tert-butylhydroquinone on the arachidonic acid metabolism in vitro

Kutil, Zsófia; Kvasnicová, Marie; Temml, V.; Schuster, D.; Vaněk, Tomáš; Fernandez, E.; Malík, J.; Landa, Přemysl

doi:https://dx.doi.org/10.1080/09540105.2014.988126

Number of the records: 1

The influence of the quinone antioxidants tert-butylhydroquinone and 2,5-di-tert-butylhydroquinone on the arachidonic acid metabolism in vitro

1.

SYSNO ASEP	0446709
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The influence of the quinone antioxidants tert-butylhydroquinone and 2,5-di-tert-butylhydroquinone on the arachidonic acid metabolism in vitro
Author(s)	Kutil, Zsófia (UEB-Q) Kvasnicová, Marie (UEB-Q)_ORCID Temml, V. (AT) Schuster, D. (AT) Vaněk, Tomáš (UEB-Q)_{RID, ORCID} Fernandez, E. (CZ) Malík, J. (CZ) Landa, Přemysl (UEB-Q)_{RID, ORCID}
Source Title	Food and Agricultural Immunology - ISSN 0954-0105 Roč. 26, č. 4 (2015), s. 504-511
Number of pages	8 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	food additives ; tert-butylhydroquinone ; cyclooxygenase
Subject RIV	CE - Biochemistry
Institutional support	UEB-Q - RVO:61389030
UT WOS	000351911800004
DOI	10.1080/09540105.2014.988126
Annotation	The aim of the present study was to elucidate the effect of the food additive tert-butylhydroquinone (TBHQ), its metabolite tert-butylbenzoquinone (TBQ), the indirect food additive 2,5-di-tert-butylhydroquinone (DTBHQ) and five other structurally related quinones on the enzymatic activity of cyclooxygenase-1 and -2 (COX-1, -2) and 5-lipoxygenase (5-LO). In a study conducted on purified enzymes TBHQ affected neither COX-1 nor COX-2 activity, but inhibited 5-LO in human neutrophils with an IC50 of 13.5 mu M. In contrast, DTBHQ regulated the activity of 5-LO as well as the activity of COX-2 (IC50 = 1.8 and 14.1 for 5-LO and COX-2, respectively). The in silico studies on DTBHQ indicated interactions with the catalytic iron of 5-LO and possible hydrogen bond interactions with His367 or His372. These data suggest that TBHQ and DTBHQ influence arachidonic acid (AA) metabolism in biologically relevant concentrations in vitro and should be further studied for their possible effect on the AA pathway in vivo.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2016

Number of the records: 1