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Disturbances in production of progesterone and their implications in plant studies
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SYSNO ASEP 0446380 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Disturbances in production of progesterone and their implications in plant studies Author(s) Janeczko, A. (PL)
Oklešťková, Jana (UEB-Q) RID, ORCID, SAI
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Sniegowska-Swierk, K. (PL)
Snaczke, Z. (PL)
Pociecha, E. (PL)Source Title Steroids. - : Elsevier - ISSN 0039-128X
Roč. 96, APR 2015 (2015), s. 153-163Number of pages 11 s. Language eng - English Country US - United States Keywords Drought ; Mifepristone ; Plant development Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000351791000019 DOI 10.1016/j.steroids.2015.01.025 Annotation Progesterone is a mammalian hormone that has also been discovered in plants but its physiological function in plants is not explained. Experiments using inhibitors of progesterone synthesis and binding would be useful in studies on the significance of this compound in plants. Until now, trilostane and mifepristone have been used in medical sciences as progesterone biosynthesis and binding inhibitors, respectively. We tested these synthetic steroids for the first time in plants and found that they reduced the content of progesterone in wheat. The aim of further experiments was to answer whether the potential disturbances in the production/binding of progesterone, influence resistance to environmental stress (drought) and the development of wheat. Inhibitors and progesterone were applied to plants via roots in a concentration of 0.25-0.5 mg/l water. Both inhibitors lowered the activity of CO2 binding enzyme (Rubisco) in wheat exposed to drought stress and trilostane additionally lowered the chlorophyll content. However, trilostane-treated plants were rescued by treatment with exogenous progesterone. The inhibitors also modulated the development of winter wheat, which indicated the significance of steroid regulators and their receptors in this process. In this study, in addition to progesterone and its inhibitors, brassinosteroid (24-epibrassinolide) and an inhibitor of biosynthesis of brassinosteroids were also applied. Mirepristone inhibited the generative development of wheat (like 24-epibrassinolide), while trilostane (like progesterone and an inhibitor of biosynthesis of brassinosteroids) stimulated the development. We propose a model of steroid-induced regulation of the development of winter wheat, where brassinosteroids act as inhibitors of generative development, while progesterone or other pregnane derivatives act as stimulators. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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