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Hyperexpansion of wheat chromosomes sorted by flow cytometry
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SYSNO ASEP 0442776 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Hyperexpansion of wheat chromosomes sorted by flow cytometry Author(s) Endo, Takashi R. (UEB-Q)
Kubaláková, Marie (UEB-Q) RID
Vrána, Jan (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCIDSource Title Genes & Genetic Systems - ISSN 1341-7568
Roč. 89, č. 4 (2014), s. 181-185Number of pages 5 s. Language eng - English Country JP - Japan Keywords flow cytometry ; flow sorting ; chromosome Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000350843100004 DOI 10.1266/ggs.89.181 Annotation Despite remarkable recent progress in the analysis of plant genome organization and chromosome structure, there is a need for methods enabling DNA sequences to be mapped by fluorescence in situ hybridization (FISH) at high spatial resolution. We sorted mitotic metaphase chromosomes of wheat by flow cytometry and observed the occurrence of hyperexpanded chromosomes among them. However, this phenomenon was not reproducible in subsequent experiments. An investigation into the procedures of flow cytometry revealed that the hyperexpansion of chromosomes became reproducible when the concentration of formaldehyde used in sample fixation was reduced. We conducted FISH analysis with 45S rDNA, 5S rDNA and wheat centromeric repeat sequences as probes on flow-sorted chromosomes and also on chromosomes from squash preparations. We measured the length of chromosomes 1B and 6B, identified by FISH. On average, the hyperexpanded 1B and 6B chromosomes were 7.26 and 7.53 times longer, respectively, than the same chromosomes from the squash preparations. The most stretched 1B and 6B chromosomes both exceeded 100 micrometers. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2015 Electronic address http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=25747042
Number of the records: 1