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Expression and epigenetic profile of protoplast cultures (Cucumis sativus L.)
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SYSNO ASEP 0441012 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Expression and epigenetic profile of protoplast cultures (Cucumis sativus L.) Author(s) Cápal, Petr (UEB-Q) RID, ORCID
Ondřej, V. (CZ)Source Title In Vitro Celluar and Developmental Biology - Plant - ISSN 1054-5476
Roč. 50, č. 6 (2014), s. 789-794Number of pages 6 s. Language eng - English Country US - United States Keywords SOMATIC EMBRYOGENESIS ; DNA METHYLATION ; CHROMATIN Subject RIV EB - Genetics ; Molecular Biology Institutional support UEB-Q - RVO:61389030 UT WOS 000346637300015 DOI 10.1007/s11627-014-9638-9 Annotation Cellular dedifferentiation is a process in which cells change from a given differentiated state into a transient, stem cell-like state and reenter into the cell cycle. In plants, cellular dedifferentiation is exemplified by protoplastization. Here, the cell wall of differentiated leaf cells is degraded by given enzymes that finish by protoplasts’ formation. The protoplasts assume a unique transient state with features resembling animal stem cells. The global chromatin decondensation is characterized for plant protoplasts that is also a distinguishing feature of animal stem cells. Various stimuli, that include phytohormones like auxins and cytokinins, induce protoplasts to reenter the cell cycle and proliferate. The reprogrammed cells could form calli, which under appropriate conditions can form shoots and roots, somatic embryos, and eventually fertile plant. However, application of auxins induces endoreduplication that could thwart future cell development; absence of these growth factors in culture of dedifferentiating protoplast cells causes their death with hallmark events of programmed cell death. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2015
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