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Induced Variations in Brassinosteroid Genes Define Barley Height and Sturdiness, and Expand the Green Revolution Genetic Toolkit
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SYSNO ASEP 0439544 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Induced Variations in Brassinosteroid Genes Define Barley Height and Sturdiness, and Expand the Green Revolution Genetic Toolkit Author(s) Dockter, C. (DK)
Gruszka, D. (PL)
Braumann, I. (DK)
Druka, A. (GB)
Franckowiak, J. (PL)
Muller, A.H. (DK)
Oklešťková, Jana (UEB-Q) RID, ORCID, SAI
Schulz, B. (US)
Zakhrabekova, S. (DK)
Hansson, M. (DK)Number of authors 18 Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 166, č. 4 (2014), s. 1912-1927Number of pages 16 s. Language eng - English Country US - United States Keywords HORDEUM-VULGARE L. ; GIBBERELLIN-SYNTHESIS ; SIGNAL-TRANSDUCTION Subject RIV EF - Botanics R&D Projects LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000346016400022 DOI 10.1104/pp.114.250738 Annotation Reduced plant height and culm robustness are quantitative characteristics important for assuring cereal crop yield and quality under adverse weather conditions. A very limited number of short-culm mutant alleles were introduced into commercial crop cultivars during the Green Revolution. We identified phenotypic traits, including sturdy culm, specific for deficiencies in brassinosteroid biosynthesis and signaling in semidwarf mutants of barley (Hordeum vulgare). This set of characteristic traits was explored to perform a phenotypic screen of near-isogenic short-culm mutant lines from the brachytic, breviaristatum, dense spike, erectoides, semibrachytic, semidwarf, and slender dwarf mutant groups. In silico mapping of brassinosteroid-related genes in the barley genome in combination with sequencing of barley mutant lines assigned more than 20 historic mutants to three brassinosteroid-biosynthesis genes (BRASSINOSTEROID-6-OXIDASE, CONSTITUTIVE PHOTOMORPHOGENIC DWARF, and DIMINUTO) and one brassinosteroid-signaling gene (BRASSINOSTEROID-INSENSITIVE1 [HvBRI1]). Analyses of F2 and M2 populations, allelic crosses, and modeling of nonsynonymous amino acid exchanges in protein crystal structures gave a further understanding of the control of barley plant architecture and sturdiness by brassinosteroid-related genes. Alternatives to the widely used but highly temperature-sensitive uzu1.a allele of HvBRI1 represent potential genetic building blocks for breeding strategies with sturdy and climate-tolerant barley cultivars. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2015
Number of the records: 1