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Induced Variations in Brassinosteroid Genes Define Barley Height and Sturdiness, and Expand the Green Revolution Genetic Toolkit

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    SYSNO ASEP0439544
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleInduced Variations in Brassinosteroid Genes Define Barley Height and Sturdiness, and Expand the Green Revolution Genetic Toolkit
    Author(s) Dockter, C. (DK)
    Gruszka, D. (PL)
    Braumann, I. (DK)
    Druka, A. (GB)
    Franckowiak, J. (PL)
    Muller, A.H. (DK)
    Oklešťková, Jana (UEB-Q) RID, ORCID, SAI
    Schulz, B. (US)
    Zakhrabekova, S. (DK)
    Hansson, M. (DK)
    Number of authors18
    Source TitlePlant Physiology. - : Oxford University Press - ISSN 0032-0889
    Roč. 166, č. 4 (2014), s. 1912-1927
    Number of pages16 s.
    Languageeng - English
    CountryUS - United States
    KeywordsHORDEUM-VULGARE L. ; GIBBERELLIN-SYNTHESIS ; SIGNAL-TRANSDUCTION
    Subject RIVEF - Botanics
    R&D ProjectsLK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportUEB-Q - RVO:61389030
    UT WOS000346016400022
    DOI10.1104/pp.114.250738
    AnnotationReduced plant height and culm robustness are quantitative characteristics important for assuring cereal crop yield and quality under adverse weather conditions. A very limited number of short-culm mutant alleles were introduced into commercial crop cultivars during the Green Revolution. We identified phenotypic traits, including sturdy culm, specific for deficiencies in brassinosteroid biosynthesis and signaling in semidwarf mutants of barley (Hordeum vulgare). This set of characteristic traits was explored to perform a phenotypic screen of near-isogenic short-culm mutant lines from the brachytic, breviaristatum, dense spike, erectoides, semibrachytic, semidwarf, and slender dwarf mutant groups. In silico mapping of brassinosteroid-related genes in the barley genome in combination with sequencing of barley mutant lines assigned more than 20 historic mutants to three brassinosteroid-biosynthesis genes (BRASSINOSTEROID-6-OXIDASE, CONSTITUTIVE PHOTOMORPHOGENIC DWARF, and DIMINUTO) and one brassinosteroid-signaling gene (BRASSINOSTEROID-INSENSITIVE1 [HvBRI1]). Analyses of F2 and M2 populations, allelic crosses, and modeling of nonsynonymous amino acid exchanges in protein crystal structures gave a further understanding of the control of barley plant architecture and sturdiness by brassinosteroid-related genes. Alternatives to the widely used but highly temperature-sensitive uzu1.a allele of HvBRI1 represent potential genetic building blocks for breeding strategies with sturdy and climate-tolerant barley cultivars.
    WorkplaceInstitute of Experimental Botany
    ContactDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Year of Publishing2015
Number of the records: 1  

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