0433402 - ÚŽFG 2015 RIV GB eng J - Journal Article
Killer, Jiří - Havlík, J. - Vlková, E. - Rada, V. - Pechar, R. - Benada, Oldřich - Kopečný, Jan - Kofroňová, Olga - Sechovcová, Hana
Lactobacillus rodentium sp nov., from the digestive tract of wild rodents.
International Journal of Systematic and Evolutionary Microbiology. Roč. 64, č. 5 (2014), 1526-1533. ISSN 1466-5026. E-ISSN 1466-5034
R&D Projects: GA ČR(CZ) GAP304/11/1252; GA ČR GA13-08803S
Institutional support: RVO:67985904 ; RVO:61388971
Keywords : Lactobacillus rodentium
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.511, year: 2014 ; AIS: 0.393, rok: 2014
DOI: https://doi.org/10.1099/ijs.0.054924-0

Three strains of regular, long, Gram-stain-positive bacterial rods were isolated using TRY, M.R.S. and Rogosa agar under anaerobic conditions from the digestive tract of wild mice (Mus musculus). All 16S rRNA gene sequences of these isolates were most similar to sequences of Lactobacillus gasseri ATCC 33323(T) and Lactobacillus johnsonii ATCC 33200(T) (97.3% and 97.2% sequence similarities, respectively). The novel strains shared 99.2-99.6% 16S rRNA gene sequence similarities. Type strains of L. gasseri and L. johnsonii were also most related to the newly isolated strains according to rpoA (83.9-84.0% similarities), pheS (84.6-87.8 %), atpA (86.2-87.7%), hsp60 (89.4-90.4%) and tuf (92.7-93.6%) gene sequence similarities. Phylogenetic studies based on 16S rRNA, hsp60, rpoA, atpA and pheS gene sequences, other genotypic and many phenotypic characteristics (results of API 50 CHL, Rapid ID 32A and API ZYM biochemical tests; cellular fatty acid profiles; cellular polar lipid profiles; end products of glucose fermentation) showed that these bacterial strains represent a novel species within the genus Lactobacillus. The name Lactobacillus rodentium sp. nov. is proposed to accommodate this group of new isolates. The type strain is MYMRS/TLU1(T) (=DSM 24759(T)=CCM 7945(T)).
Permanent Link: http://hdl.handle.net/11104/0237680