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Proteome Analysis of Cold Response in Spring and Winter Wheat (Triticum aestivum) Crowns Reveals Similarities in Stress Adaptation and Differences in Regulatory Processes between the Growth Habits
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SYSNO ASEP 0421944 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Proteome Analysis of Cold Response in Spring and Winter Wheat (Triticum aestivum) Crowns Reveals Similarities in Stress Adaptation and Differences in Regulatory Processes between the Growth Habits Author(s) Kosová, K. (CZ)
Vítámvás, P. (CZ)
Planchon, S. (LU)
Renaut, J. (LU)
Vaňková, Radomíra (UEB-Q) RID, ORCID
Prášil, I.T. (CZ)Source Title Journal of Proteome Research. - : American Chemical Society - ISSN 1535-3893
Roč. 12, č. 11 (2013), s. 4830-4845Number of pages 16 s. Language eng - English Country US - United States Keywords 2D-DIGE analysis ; cold stress ; spring and winter growth habit Subject RIV ED - Physiology R&D Projects GA522/09/2058 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000326615000015 DOI 10.1021/pr400600g Annotation A proteomic response to cold treatment (4 degrees C) has been studied in crowns of a frost-tolerant winter wheat cultivar Samanta and a frost-sensitive spring wheat cultivar Sandra after short-term (3 days) and long-term (21 days) cold treatments. Densitometric analysis of 2-D differential in gel electrophoresis (2D-DIGE) gels has resulted in the detection of 386 differentially abundant protein spots, which reveal at least a two-fold change between experimental variants. Of these, 58 representative protein spots have been selected for MALDI-TOF/TOF identification, and 36 proteins have been identified. The identified proteins with an increased relative abundance upon cold in both growth habits include proteins involved in carbohydrate catabolism (glycolysis enzymes), redox metabolism (thioredoxin-dependent peroxidase), chaperones, as well as defense-related proteins (protein revealing similarity to thaumatin). Proteins exhibiting a cold-induced increase in the winter cultivar include proteins involved in regulation of stress response and development (germin E, lectin VER2), while proteins showing a cold-induced increase in the spring cultivar include proteins involved in restoration of cell division and plant growth (eIF5A2, glycine-rich RNA-binding protein, adenine phosphoribosyltransferase). These results provide new insights into cold acclimation in spring and winter wheat at the proteome level and enrich our previous work aimed at phytohormone dynamics in the same plant material. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2014
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