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In Vivo Determination of Organellar pH Using a Universal Wavelength-Based Confocal Microscopy Approach
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SYSNO ASEP 0377982 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title In Vivo Determination of Organellar pH Using a Universal Wavelength-Based Confocal Microscopy Approach Author(s) Rodó, A. P. (CZ)
Váchová, Libuše (MBU-M) RID, ORCID
Palková, Z. (CZ)Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 7, č. 3 (2012), s. 1-12Number of pages 12 s. Language eng - English Country US - United States Keywords GREEN FLUORESCENT PROTEIN ; YEAST COLONY DEVELOPMENT ; INTRACELLULAR PH Subject RIV EE - Microbiology, Virology R&D Projects GA204/08/0718 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000303857100023 DOI 10.1371/journal.pone.0033229 Annotation Many essential cellular processes are affected by transmembrane H+ gradients and intracellular pH (pHi). The research of such metabolic events calls for a non-invasive method to monitor pHi within individual subcellular compartments. We present a novel confocal microscopy approach for the determination of organellar pHi in living cells expressing pHdependent ratiometric fluorescent proteins. Unlike conventional intensity-based fluorometry, our method relies on emission wavelength scans at single-organelle resolution to produce wavelength-based pH estimates both accurate and robust to low-signal artifacts Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2013
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