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Cytokinins in shoot apices of Brassica napus plants during vernalization
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SYSNO ASEP 0375184 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cytokinins in shoot apices of Brassica napus plants during vernalization Author(s) Tarkowská, Danuše (UEB-Q) RID, ORCID
Filek, M. (PL)
Biesaga-Koscielniak, J. (PL)
Marcinska, I. (PL)
Macháčková, Ivana (UEB-Q)
Krekule, Jan (UEB-Q)
Strnad, Miroslav (UEB-Q) RID, ORCIDNumber of authors 7 Source Title Plant Science. - : Elsevier - ISSN 0168-9452
Roč. 187, č. 1 (2012), s. 105-112Number of pages 8 s. Language eng - English Country IE - Ireland Keywords Cytokinins ; Vernalization ; Winter rape ; Flowering Subject RIV EF - Botanics R&D Projects KAN200380801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) ED0007/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) UT WOS 000302489700012 DOI 10.1016/j.plantsci.2012.02.003 Annotation The putative role of cytokinins in processes leading to reproductive development of plants was investigated by analysing the shoot apical parts of a winter cultivar of oilseed rape (Brassica napus L. var. oleifera, cv. Górczański). The endogenous cytokinin levels were measured by liquid chromatography–tandem mass spectrometry (LC–MS) in the shoot apices of vegetative plants (grown at 20/17 °C with a 16/8 h day/night regime) and vernalized plants (56 days at 5/2 °C with a 16/8 h photoperiod) at different times during floral transition. During vernalization, the content of all isoprenoid cytokinins increased significantly, coinciding well with the onset of the early stages of reproductive development. Cytokinin levels reached their maxima when most of the plants became irreversibly reproductive (after 42 days of cold treatment). cis-Zeatin riboside (unequivocally identified by quadrupole-time-of-flight MS) accounted for ca. 87–89% of the total isoprenoid cytokinin content in control and vernalized plants, whilst N6-isopentenyladenosine (6% in control and 8% in vernalized plants) and cis-zeatin (2% in control and 1% in vernalized plants) were the next most abundant cytokinins. In the post-vernalization period, endogenous cytokinin levels decreased, but remained significantly higher in the reproductive plants than in the vegetative controls. These results suggest that cytokinins, especially those of the cis-zeatin type, are involved in vernalization-induced reproductive development of B. napus. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2012
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