Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures

Vondráková, Zuzana; Cvikrová, Milena; Eliášová, Kateřina; Martincová, Olga; Vágner, Martin

doi:https://dx.doi.org/10.1093/treephys/tpq074

Number of the records: 1

Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures

1.

SYSNO ASEP	0357035
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures
Author(s)	Vondráková, Zuzana (UEB-Q)_{RID, ORCID} Cvikrová, Milena (UEB-Q)_{RID, ORCID} Eliášová, Kateřina (UEB-Q)_{RID, ORCID} Martincová, Olga (UEB-Q) Vágner, Martin (UEB-Q)_{RID, ORCID}
Source Title	Tree Physiology - ISSN 0829-318X Roč. 30, č. 10 (2010), s. 1335-1348
Number of pages	14 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	cryopreservation ; Norway spruce (Picea abies (L ) Karst) ; somatic embryogenesis
Subject RIV	EA - Cell Biology
R&D Projects	OC 158 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	OC08013 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000282818100009
DOI	10.1093/treephys/tpq074
Annotation	The anatomical characteristics and PA content during cryopreservation and regrowth were studied in the ESMs of AFO 541 and C110 cultures, which have comparable ESM anatomy but diverse growth rates, PA content and regeneration abilities after cryopreservation. The endogenous free PAs were maintained at high levels, with spermidine being the predominant PA in the AFO 541, while in C110 the content of putrescine and spermidine was almost identical and rather low, the content of spermidine being approximately one-third that in the ESM of AFO 541. Osmotic pretreatment, using a double application of sorbitol followed by an application of dimethyl sulfoxide (DMSO) resulted in the continual disintegration of polyembryogenic centers and suspensors in both cell lines. A continual decrease in the level of PAs was observed during the cell osmotic pretreatment. The cells that retained their viability and regrowth ability after cryopreservation were the meristematic cells inside embryonal heads.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1