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Identification of Staphylococcus spp. isolated from ixodid ticks
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SYSNO ASEP 0315074 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Identification of Staphylococcus spp. isolated from ixodid ticks Title Identifikace stafylokoků izolovaných z klíšťat Author(s) Švec, P. (CZ)
Nováková, D. (CZ)
Rudolf, Ivo (UBO-W) RID, ORCID, SAI
Šikutová, Silvie (UBO-W) RID, SAI, SAI, ORCID
Masaříková, J. (CZ)
Hubálek, Zdeněk (UBO-W) RID, SAI, ORCID
Sedláček, I. (CZ)Source Title XII. International Congress of Bacteriology and Applied Microbiology, 5-9 August 2008, Istanbul: Abstract Book. - Istanbul : International Union of Microbiological Societies, 2008
S. 268Number of pages 1 s. Action International Congress of Bacteriology and Applied Microbiology /12./ Event date 05.08.2008-09.08.2008 VEvent location Istanbul Country TR - Turkey Event type WRD Language eng - English Country TR - Turkey Keywords ticks ; diversity ; microorganisms ; rep-PCR ; staphylococci Subject RIV EE - Microbiology, Virology R&D Projects KJB600930613 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z60930519 - UBO-W (2005-2011) Annotation A group of eight Gram-positive, catalase positive cocci was isolated during analysis of cultivable microflora of ixodid ticks, namely Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna, vectors of many vertebrate pathogens. Analysed strains were isolated from whole-body homogenized surface-sterilized ticks inoculated on different kinds of media: Tryptone Soya Agar, Brain-Heart Infusion Agar, Columbia Blood Agar and Mac Conkey Agar. Individual colonies were picked up, purified and preliminary identified by using rep-PCR fingerprinting with the primer (GTG)5. This method assigned three strains as Staphylococcus aureus subsp. aureus (DR11, HC37, HC48), two strains as Staphylococcus warneri (HC13, HC36) and the remaining strains represented Staphylococcus cohnii subsp. cohnii (DR8), Staphylococcus epidermidis (HC34) and Staphylococcus capitis subsp. capitis (HC31). These results were verified by automated ribotyping with EcoRI (RiboPrinter® microbial characterization system) as well as whole-cell protein fingerprinting and confirmed the (GTG)5-PCR fingerprinting as a fast and reliable tool for identification of staphylococci. Biotyping using API ID 32 Staph kit misidentified S. cohnii subsp. cohnii (DR8) as Staphylococcus xylosus and S. warneri (HC13) as Staphylococcus haemolyticus. Two strains (DR11 and DR8) originated from Dermacentor reticulatus and the remaining strains were isolated from Haemaphysalis concinna. Interestingly, no staphylococci were isolated from Ixodes ricinus in our study. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2009
Number of the records: 1