Does DNA methylation pattern mark generative development in winter rape?
1.
SYSNO ASEP
0082520
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Does DNA methylation pattern mark generative development in winter rape?
Title
Does DNA methylation pattern mark generative development in winter rape?
Author(s)
Filek, M. (PL) Janiak, A. (PL) Szarejko, I. (PL) Grabczynska, J. (PL) Macháčková, Ivana (UEB-Q) Krekule, Jan (UEB-Q)
Source Title
Zeitschrift fuer Naturforschung. Section C: a Journal of Biosciences. - : Walter de Gruyter
- ISSN 0939-5075
Roč. 61, 5-6 (2006), s. 387-396
Number of pages
10 s.
Language
eng - English
Country
DE - Germany
Keywords
DNA methylation ; rape ; vernalization
Subject RIV
EF - Botanics
R&D Projects
IAA600040612 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ
AV0Z50380511 - UEB-Q (2005-2011)
Annotation
In this paper we report on changes in DNA methylation pattern in rape apices and leaves during transition from vegetative to reproductive stage due to grafting and/or vernalization. Grafted plants of winter rape (Brassica napus L., var. "Gorczanski") (stock from vernalized, scion from non-vernalized plants) were used together with vernalized non-grafted plants. In addition, methylation status was determined also in spring rape (var. "Mlochowski") grown under normal and low temperature. The methylation-sensitive amplification polymorphism (MSAP) method with EcoRI/MspI and EcoRII/HpaII restriction enzymes was employed. The majority (ca. 68%) of analyzed loci (566 in winter and 551 in spring rape) were monomorphic, i.e. did not undergo methylation. Both cultivars showed a similar degree of methylation. 188 loci in winter and 176 in spring cultivars expressed changes in the methylation pattern. All differentially amplified fragments resulted from either full methylation of an internal cytosine or from hemi-methylation of an external cytosine. A pair-wise comparison showed that a similar number of loci underwent development-related methylation changes in apices of the winter and spring rape. The majority (80%) of changes were demethylation events in generative (vernalized) apices of the winter cultivar. However, an increased number of demethylated loci was detected in vernalized apices in comparison with generative, non-vernalized ones. In apices of vegetative and generative grafted plants the same number of demethylation events was observed. Overall, 10 MSAP loci were detected that expressed methylation changes in vernalized apices only; among them 7 loci underwent demethylation after vernalization and remained methylated in both vegetative and generative non-vernalized stage. Only 1 locus was demethylated in generative non-vernalized apices. Thus, most of demethylation events can be ascribed to vernalization and not to the generative stage. In leaves of winter rape methylation and demethylation events occurred with similar frequency, while in the spring cultivar more demethylation events were detected. The results show that during vernalization and transition to the generative stage different sets of genes are activated.
Workplace
Institute of Experimental Botany
Contact
David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing
2007
Number of the records: 1
This site uses cookies to make them easier to browse. Learn more about
how we use cookies.
