Number of the records: 1
Bacterial response to nanodiamonds and graphene oxide sheets
- 1.0470429 - FZU-D 2017 RIV DE eng J - Journal Article
Kromka, Alexander - Jíra, Jaroslav - Štenclová, Pavla - Kříha, V. - Kozak, Halyna - Beranová, J. - Vretenár, V. - Skakalova, V. - Rezek, Bohuslav
Bacterial response to nanodiamonds and graphene oxide sheets.
Physica Status Solidi B-Basic Solid State Physics. Roč. 253, č. 12 (2016), 2481-2485. ISSN 0370-1972
R&D Projects: GA MŠk LM2015087; GA ČR GA15-01687S
Institutional support: RVO:68378271
Keywords : diamond nanoparticles * graphene oxide * antibacterial properties * Escherichia coli
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.674, year: 2016
We report on antibacterial activity of NDs and GO as evaluated by bacterial colony counting. ND and GO were used in oxidized (O-ND, GO) or reduced (H-ND, rGO) forms. ND or GO are mixed in Mueller-Hinton (MH) broth with Escherichia coli at the concentration of 1 mg/ml. The resulting suspensions were cultivated for 5 and 24 h at 37°C in a shaker. After each period a sample of the suspension was spread on the MH agar and cultivated for 24 h at 37° C. The effect of nanomaterials on colony forming ability (CFA) of E. coli was evaluated compared to negative control sample. The most noticeable effect was that after 5 hours H-ND decreased the CFA of E. coli in the MH broth by 60% and GO by 50%. After 24 hours, H-ND decreased CFA by 50%. Other nanomaterials had no statistically significant antibacterial effect. Factors influencing ND and GO antibacterial activity against the E. coli and possibly against other bacteria are discussed.
Permanent Link: http://hdl.handle.net/11104/0268074