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The phosphatidylcholine-hydrolysing phospholipase C NPC4 plays a role in response of Arabidopsis roots to salt stress

  1. 1. 0369946 - UEB-Q 2012 RIV GB eng J - Journal Article
    Kocourková, Daniela - Krčková, Zuzana - Pejchar, Přemysl - Veselková, Štěpánka - Valentová, O. - Wimalasekera, R. - Scherer, G. F. E. - Martinec, Jan
    The phosphatidylcholine-hydrolysing phospholipase C NPC4 plays a role in response of Arabidopsis roots to salt stress.
    Journal of Experimental Botany. Roč. 62, č. 11 (2011), s. 3753-3763 ISSN 0022-0957
    R&D Projects: GA ČR GA522/07/1614; GA MŠk(CZ) LC06034
    Institutional research plan: CEZ:AV0Z50380511
    Keywords : Arabidopsis thaliana * diacylglycerol * phospholipase C
    Subject RIV: ED - Physiology
    Impact factor: 5.364, year: 2011

    Phosphatidylcholine-hydrolysing phospholipase C, also known as non-specific phospholipase C is a new member of the plant phospholipase family that reacts to environmental stresses such as phosphate deficiency and aluminium toxicity, and has a role in root development and brassinolide signalling. Expression of NPC4, one of the six NPC genes in Arabidopsis, was highly induced by NaCl. Maximum expression was observed from 3 h to 6 h after the salt treatment and was dependent on salt concentration. Results of histochemical analysis of PNPC4:GUS plants showed the localization of salt-induced expression in root tips. On the biochemical level,increased NPC enzyme activity, indicated by accumulation of diacylglycerol, was observed as early as after 30 min of salt treatment of Arabidopsis seedlings. Phenotype analysis of NPC4 knockout plants showed increased sensitivity to salinity as compared with wildtype plants.These observations demonstrate a role for NPC4 in the response of Arabidopsis to salt stress.
    Permanent Link: http://hdl.handle.net/11104/0203887
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