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The role of auxins in somatic embryogenesis of Abies alba

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    0367372 - ÚEB 2012 RIV PL eng J - Journal Article
    Vondráková, Zuzana - Eliášová, Kateřina - Fischerová, Lucie - Vágner, Martin
    The role of auxins in somatic embryogenesis of Abies alba.
    Central European Journal of Biology. Roč. 6, č. 4 (2011), s. 587-596. ISSN 1895-104X
    R&D Projects: GA MŠk OC 158
    Institutional research plan: CEZ:AV0Z50380511
    Keywords : Auxin inhibitor * Fir * Phytohormone
    Subject RIV: EF - Botanics
    Impact factor: 1.000, year: 2011

    We report the effects of the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the auxin inhibitor p-chlorophenoxyisobutyric acid (PCIB) on the endogenous level of the auxin indole-3-acetic acid (IAA) and on the anatomical composition of early somatic embryos of Abies alba (European silver fir). The embryogenic suspensor mass (ESM) of Abies alba proliferated on a medium supplemented by 2,4-D as well as on an auxin-free medium. The endogenous level of IAA was significantly higher in the ESM cultivated on a medium supplemented by 2,4-D. The decrease in the endogenous level of IAA in the first week of maturation is one of the most important stimuli responsible for the subsequent development of embryos. Suppression of IAA synthesis by an auxin inhibitor did not stimulate the development of embryos. The maturation of somatic embryos from the globular to the cotyledonary stage occurs when the concentration of endogenous auxin in the ESM (including the embryos) increases.
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