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The osmotolerant fructophilic yeast Zygosaccharomyces rouxii employs two plasma-membrane fructose uptake systems belonging to a new family of yeast sugar transporters
0358896 - FGU-C 2012 RIV GB eng J - Journal Article
Leandro, Maria José - Sychrová, Hana - Prista, C. - Loureiro-Dias, M.C.
The osmotolerant fructophilic yeast Zygosaccharomyces rouxii employs two plasma-membrane fructose uptake systems belonging to a new family of yeast sugar transporters.
Microbiology. Roč. 157, č. 2 (2011), s. 601-608 ISSN 1350-0872
R&D Projects: GA MŠk(CZ) LC531; GA ČR(CZ) GAP503/10/0307
Institutional research plan: CEZ:AV0Z50110509
Keywords : Zygosaccharomyces rouxii * fructose transporter * heterologous expression * Saccharomyces cerevisiae
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.061, year: 2011
We have identified two genes encoding putative fructose transporters in the Z. rouxii CBS 732 genome. Heterologous expression of the two Z. rouxii ORFs in a S. cerevisiae strain lacking its own hexose transporters (hxt-null) and subsequent kinetic analysis of sugar transport showed that both proteins are functionally expressed at the plasma-membrane, ZrFfz1 being a high capacity fructose-specific facilitator and ZrFfz2 a facilitator transporting glucose and fructose with similar capacity and affinity
Permanent Link: http://hdl.handle.net/11104/0196808