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AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes

  1. 1. 0329884 - UZFG-Y 2010 RIV GB eng J - Journal Article
    Kalous, Jaroslav - Kubelka, Michal - Šolc, Petr - Šušor, Andrej - Motlík, Jan
    AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes.
    Reproduction. Roč. 138, - (2009), s. 645-654 ISSN 1470-1626
    R&D Projects: GA ČR GA204/06/1297; GA ČR GA523/03/0857; GA ČR GA524/07/1087
    Institutional research plan: CEZ:AV0Z50450515
    Keywords : protein kinase * porcine oocyte * oocyte maturation
    Subject RIV: CE - Biochemistry
    Impact factor: 2.579, year: 2009

    The aim of this study was to investigate the involvement of the serine/threonine protein kinase AKT (also called protein kinase B) in the control of meiosis of porcine denuded oocytes (DOs) matured in vitro. Western blot analysis revealed that the two principal AKT phosphorylation sites, Ser473 and Thr308, are phosphorylated at different stages of meiosis. In freshly isolated germinal vesicle (GV)-stage DOs, Ser473 was already phosphorylated. After the onset of oocyte maturation, the intensity of the Ser473 phosphorylation increased, however, which declined sharply when DOs underwent GV breakdown (GVBD) and remained at low levels in metaphase I- and II-stage (MI- and MII-stage). In contrast, phosphorylation of Thr308 was increased by the time of GVBD and reached maximum at MI-stage. A peak of AKT activity was noticed around GVBD and activity of AKT declined at MI-stage. To assess the role of AKT during meiosis, porcine DOs were cultured in 50 mikroM SH-6, a specific
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