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Immobilization of L-glyceryl phosphorylcholine: isolation of phosphorylcholine-binding proteins from seminal plasma

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    0191443 - UMG-J 20023016 RIV IE eng J - Journal Article
    Liberda, J. - Maňásková, Pavla - Švesták, M. - Jonáková, Věra - Tichá, M.
    Immobilization of L-glyceryl phosphorylcholine: isolation of phosphorylcholine-binding proteins from seminal plasma.
    Journal of Chromatography-B. Roč. 770, 1-2 (2002), s. 101-110. ISSN 0378-4347
    R&D Projects: GA ČR GA303/99/0357; GA ČR GV524/96/K162
    Institutional research plan: CEZ:AV0Z5052915; CEZ:MSM 113100001
    Keywords : L-glyceryl phosphorylcholine * proteins
    Subject RIV: EB - Genetics ; Molecular Biology
    Impact factor: 1.913, year: 2002

    The preparation of an affinity sorbent containing immobilized L-glyceryl phosphorylcholine for affinity chromatography of phosphorylcholine-binding proteins from bear, bull and human seminal plasmas is described. Adsorbed phosphorylcholine-binding proteins were characterized by SDS-PAGE and RP HPLC. In human seminal plasma no phosphorylcholine-binding proteins were found.
    Permanent Link: http://hdl.handle.net/11104/0087195