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Optimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cells
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SYSNO ASEP 0617182 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve SCOPUS Title Optimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cells Author(s) Krobot, Š. (CZ)
Menčík, P. (CZ)
Chaloupková, K. (CZ)
Bočkaj, J. (SK)
Vach Agócsová, S. (SK)
Klusáček Rampichová, Michala (UEM-P) RID, ORCID
Hedvičáková, Věra (UEM-P)
Alexy, P. (SK)
Přikryl, R. (CZ)
Melcová, V. (CZ)Number of authors 10 Source Title International Journal of Bioprinting - ISSN 2424-7723
Roč. 11, č. 1 (2025), s. 400-417Number of pages 18 s. Language eng - English Country SG - Singapore Keywords 3D printing ; design of experiment ; fused deposition modeling ; Poly(3-hydroxybutyrate) ; Polylactide ; printability ; scaffold OECD category Biomaterials (as related to medical implants, devices, sensors) R&D Projects EH22_008/0004562 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEM-P - RVO:68378041 EID SCOPUS 85218863490 DOI https://doi.org/10.36922/ijb.5175 Annotation Bone tissue engineering requires scaffolds with three-dimensional (3D) structures that facilitate vascularization and new tissue growth. 3D printing, especially through fused deposition modeling (FDM), has emerged as an effective method for creating complex structures with high reproducibility. Early research in this area demonstrated the potential of poly(ε-caprolactone) (PCL) and poly(L-lactide) (PLLA) scaffolds for bone regeneration. Recently, polylactide (PLA) and polyhydroxyalkanoates (PHAs) have garnered attention for their biocompatibility and ability to support cell proliferation. Among PHAs, poly(3-hydroxybutyrate) (PHB) shows promise due to its intrinsic biocompatibility and resorbability, making it a candidate for FDM-based scaffold fabrication. In the presented study, we aim to develop and optimize a biocompatible PHB-based composite material for bone tissue engineering, incorporating PLA, hydroxyapatite, and the plasticizer Syncroflex 3114 to enhance mechanical properties and printability. This composite was processed into filaments for 3D printing and characterized through thermal, mechanical, and biological evaluations. Using a design of experiment approach, we investigated factors such as temperature performance, warping, degradation, and strength to determine the optimal composition for use in tissue engineering. Four optimal mixture compositions fulfilling the optimization criteria of having the most suitable properties for bone tissue engineering, namely the best printability and maximum mechanical properties, were obtained. The mixtures were optimized specifically for minimum warping coefficient (0.5), maximum flexural strength (66.9 MPa), maximum compression modulus (2.4 GPa), and maximum compression modulus (2.3 GPa) with a warping coefficient of no more than 1 at the same time. In conclusion, the study shows a new possible way to effectively develop and test 3D-printed PHB-based scaffolds with specifically optimized material properties. Workplace Institute of Experimental Medicine Contact Arzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2026 Electronic address https://accscience.com/journal/IJB/articles/online_first/4158
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