Number of the records: 1
Nuclear patterns of phosphatidylinositol 4,5-and 3,4-bisphosphate revealed by super-resolution microscopy differ between the consecutive stages of RNA polymerase II transcription
- 1.
SYSNO ASEP 0601091 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nuclear patterns of phosphatidylinositol 4,5-and 3,4-bisphosphate revealed by super-resolution microscopy differ between the consecutive stages of RNA polymerase II transcription Author(s) Hoboth, Peter (UMG-J) ORCID
Sztacho, Martin (UMG-J) ORCID
Hozák, Pavel (UMG-J) RID, ORCIDNumber of authors 3 Source Title FEBS Journal - ISSN 1742-464X
Roč. 291, č. 19 (2024), s. 4240-4264Number of pages 25 s. Language eng - English Country GB - United Kingdom Keywords swiss 3t3-cells ; phospholipase-c ; ctd ; phosphorylation ; speckles ; phosphoinositides ; kinase ; cells ; organization ; association ; gene expression ; nuclear architecture ; nuclear speckles ; nucleoplasm ; quantitative direct stochastic optical reconstruction microscopy dSTORM OECD category Cell biology R&D Projects LM2023050 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LX22NPO5102 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC19048 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC20024 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 001219071800001 EID SCOPUS 85193002640 DOI https://doi.org/10.1111/febs.17136 Annotation Phosphatidylinositol phosphates are powerful signaling molecules that orchestrate signaling and direct membrane trafficking in the cytosol. Interestingly, phosphatidylinositol phosphates also localize within the membrane-less compartments of the cell nucleus, where they participate in the regulation of gene expression. Nevertheless, current models of gene expression, which include condensates of proteins and nucleic acids, do not include nuclear phosphatidylinositol phosphates. This gap is partly a result of the missing detailed analysis of the subnuclear distribution of phosphatidylinositol phosphates and their relationships with gene expression. Here, we used quantitative dual-color direct stochastic optical reconstruction microscopy to analyze the nanoscale co-patterning between RNA polymerase II transcription initiation and elongation markers with respect to phosphatidylinositol 4,5- or 3,4-bisphosphate in the nucleoplasm and nuclear speckles and compared it with randomized data and cells with inhibited transcription. We found specific co-patterning of the transcription initiation marker P-S5 with phosphatidylinositol 4,5-bisphosphate in the nucleoplasm and with phosphatidylinositol 3,4-bisphosphate at the periphery of nuclear speckles. We showed the specific accumulation of the transcription elongation marker PS-2 and of nascent RNA in the proximity of phosphatidylinositol 3,4-bisphosphate associated with nuclear speckles. Taken together, this shows that the distinct spatial associations between the consecutive stages of RNA polymerase II transcription and nuclear phosphatidylinositol phosphates exhibit specificity within the gene expression compartments. Thus, in analogy to the cellular membranes, where phospholipid composition orchestrates signaling pathways and directs membrane trafficking, we propose a model in which the phospholipid identity of gene expression compartments orchestrates RNA polymerase II transcription. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2025 Electronic address https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.17136
Number of the records: 1