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Ectopic assembly of an auxin efflux control machinery shifts developmental trajectories
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SYSNO ASEP 0601087 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ectopic assembly of an auxin efflux control machinery shifts developmental trajectories Author(s) Fandino, A. C. A. (CH)
Jelínková, Adriana (UEB-Q) RID, ORCID
Marhava, P. (CH)
Petrášek, Jan (UEB-Q) RID, ORCID
Hardtke, C.S. (CH)Number of authors 5 Source Title Plant Cell. - : Oxford University Press - ISSN 1040-4651
Roč. 36, č. 5 (2024), s. 1791-1805Number of pages 15 s. Language eng - English Country US - United States Keywords plasma-membrane ; transport ; polarity ; phosphorylation ; endocytosis ; kinases ; growth ; trafficking ; expression ; carriers OECD category Developmental biology R&D Projects GF21-08021L GA ČR - Czech Science Foundation (CSF) Research Infrastructure Czech-BioImaging III - 90250 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 001170000100001 EID SCOPUS 85192027509 DOI https://doi.org/10.1093/plcell/koae023 Annotation Polar auxin transport in the Arabidopsis (Arabidopsis thaliana) root tip maintains high auxin levels around the stem cell niche that gradually decrease in dividing cells but increase again once they transition toward differentiation. Protophloem differentiates earlier than other proximal tissues and employs a unique auxin <canalization> machinery that is thought to balance auxin efflux with retention. It consists of a proposed activator of PIN-FORMED (PIN) auxin efflux carriers, the cAMP-, cGMP- and Calcium-dependent (AGC) kinase PROTEIN KINASE ASSOCIATED WITH BRX (PAX), its inhibitor, BREVIS RADIX (BRX), and PHOSPHATIDYLINOSITOL-4-PHOSPHATE-5-KINASE (PIP5K) enzymes, which promote polar PAX and BRX localization. Because of a dynamic PAX-BRX-PIP5K interplay, the net cellular output of this machinery remains unclear. In this study, we deciphered the dosage-sensitive regulatory interactions among PAX, BRX, and PIP5K by their ectopic expression in developing xylem vessels. The data suggest that the dominant collective output of the PAX-BRX-PIP5K module is a localized reduction in PIN abundance. This requires PAX-stimulated clathrin-mediated PIN endocytosis upon site-specific phosphorylation, which distinguishes PAX from other AGC kinases. An ectopic assembly of the PAX-BRX-PIP5K module is sufficient to cause cellular auxin retention and affects root growth vigor by accelerating the trajectory of xylem vessel development. Our data thus provide direct evidence that local manipulation of auxin efflux alters the timing of cellular differentiation in the root. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2025 Electronic address https://doi.org/10.1093/plcell/koae023
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