0599652 - ÚEB 2025 RIV CN eng J - Journal Article
Nowicka, Anna - Kováčik, Martin - Maksylewicz, A. - Kopeć, P. - Dubas, E. - Krzewska, M. - Springer, A. - Hoffie, R. E. - Daghma, D. S. - Milec, Zbyněk - Pečinka, Aleš - Kumlehn, J. - Żur, I.
The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley.
Crop Journal. Roč. 12, č. 4 (2024), s. 1064-1080. ISSN 2095-5421. E-ISSN 2214-5141
R&D Projects: GA MŠMT(CZ) EH22_008/0004581; GA MŠMT(CZ) EH22_008/0004635; GA ČR(CZ) GA21-02929S
Institutional support: RVO:61389030 ; RVO:86652079
Keywords : gene-expression * embryogenesis * embryo * differentiation * identification * trichostatin * methylation * totipotency * metabolism * tolerance * Hordeum vulgare * Marker gene * Microspore embryogenesis * Stress response * Transcriptome analysis
OECD category: Cell biology
Impact factor: 6, year: 2023 ; AIS: 0.965, rok: 2023
Method of publishing: Open access
Result website:
https://doi.org/10.1016/j.cj.2024.07.003DOI: https://doi.org/10.1016/j.cj.2024.07.003

Plant formation from in vitro-cultivated microspores involves a complex network of internal and environmental factors. Haploids/doubled haploids (DHs) derived from in vitro-cultured microspores are widely used in plant breeding and genetic engineering. However, the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly defined. Here, RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis (ME) in barley cultivars Golden Promise and Igri, which exhibit contrasting responsiveness to microspore-derived plant formation. Our experiments revealed fundamental regulatory networks, specific groups of genes, and transcription factor (TF) families potentially regulating the developmental switch. We identified a set of candidate genes crucial for genotype-dependent responsiveness/recalcitrance to ME. Our high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.
Permanent Link: https://hdl.handle.net/11104/0357100


 
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