0587846 - FGÚ 2025 RIV CZ eng J - Journal Article
Rauchová, Hana - Neprašová, B. - Maletínská, L. - Kuneš, Jaroslav
Glutathione Levels and Lipid Oxidative Damage in Selected Organs of Obese Koletsky and Lean Spontaneously Hypertensive Rats.
Physiological Research. Roč. 73, č. 3 (2024), s. 481-484. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA MŠMT(CZ) LX22NPO5104
Institutional support: RVO:67985823
Keywords : thiobarbituric acid-reactive substances * heart * renal cortex * renal medulla * liver
OECD category: Cardiac and Cardiovascular systems
Impact factor: 1.9, year: 2023 ; AIS: 0.394, rok: 2023
Method of publishing: Open access
Result website:
https://www.biomed.cas.cz/physiolres/pdf/2024/73_481.pdf

DOI: https://doi.org/10.33549/physiolres.935319

Koletsky rats, the genetically obese strain of spontaneously hypertensive rats (SHROB), are the well-accepted animal model of human metabolic syndrome. They are characterized by early onset obesity, spontaneous hypertension, hyperinsulinemia, hyperlipidemia, proteinuria and shortened life-span. One of the factors in the pathogenesis of metabolic syndrome is oxidative stress. The aim of the present study was to compare two parameters related to oxidative stress: the levels of the main intracellular antioxidant, reduced glutathione as well as the indirect indicator of lipid peroxidation damage, thiobarbituric acid-reactive substances (TBARS) in heart, renal cortex and medulla and liver in male lean spontaneously hypertensive rats (SHR) and obese Koletsky rats. We did not find any significant differences in these markers in heart and kidneys. However, we found significantly lower glutathione level in Koletsky rat liver compared with SHR (5.03 +/- 0.23 vs. 5.83 +/- 0.14 mu mol/g tissue, respectively). On the contrary, we observed significantly higher TBARS levels in Koletsky rat liver compared with SHR (28.56 +/- 2.15 vs. 21.83 +/- 1.60 nmol/mg protein, respectively). We conclude that the liver is the most sensitive tissue to oxidative damage with the significantly decreased concentration of glutathione and the significantly increased concentration of TBARS in obese Koletsky rats in comparison with lean control SHR.
Permanent Link: https://hdl.handle.net/11104/0355362