Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance

0587240 - ÚEB 2025 RIV US eng J - Journal Article
Lu, C. - Du, J. - Chen, H. - Gong, S. - Jin, Y. - Meng, X. - Zhang, T. - Fu, B. - Molnár, István - Holušová, Kateřina - Said, Mahmoud - Xing, L. - Kong, L. - Doležel, Jaroslav - Li, G. - Wu, J. - Chen, P. - Cao, A. - Zhang, R.
Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance.
Nature Communications. Roč. 15, č. 1 (2024), č. článku 503. E-ISSN 2041-1723
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : developmental-stage * gene * pm3 * pathogens * rye * chromosome * expression * effector * quality * confer
OECD category: Biochemistry and molecular biology
Impact factor: 16.6, year: 2022
Method of publishing: Open access
https://doi.org/10.1038/s41467-024-44796-0

Powdery mildew poses a significant threat to wheat crops worldwide, emphasizing the need for durable disease control strategies. The wheat-Dasypyrum villosum T5AL<middle dot>5 V#4 S and T5DL<middle dot>5 V#4 S translocation lines carrying powdery mildew resistant gene Pm55 shows developmental-stage and tissue-specific resistance, whereas T5DL<middle dot>5 V#5 S line carrying Pm5V confers resistance at all stages. Here, we clone Pm55 and Pm5V, and reveal that they are allelic and renamed as Pm55a and Pm55b, respectively. The two Pm55 alleles encode coiled-coil, nucleotide-binding site-leucine-rich repeat (CNL) proteins, conferring broad-spectrum resistance to powdery mildew. However, they interact differently with a linked inhibitor gene, SuPm55 to cause different resistance to wheat powdery mildew. Notably, Pm55 and SuPm55 encode unrelated CNL proteins, and the inactivation of SuPm55 significantly reduces plant fitness. Combining SuPm55/Pm55a and Pm55b in wheat does not result in allele suppression or yield penalty. Our results provide not only insights into the suppression of resistance in wheat, but also a strategy for breeding durable resistance.
Permanent Link: https://hdl.handle.net/11104/0354474