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Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting
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SYSNO ASEP 0586459 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting Author(s) Fojtík, Lukáš (MBU-M) ORCID
Kalaninová, Zuzana (MBU-M) ORCID, RID
Fiala, Jan (MBU-M) ORCID, RID
Halada, Petr (MBU-M) RID, ORCID
Chmelík, Josef (MBU-M) RID, ORCID
Man, Petr (MBU-M) RID, ORCID
Kukačka, Zdeněk (MBU-M) RID, ORCID
Novák, Petr (MBU-M) RID, ORCIDSource Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 96, č. 19 (2024), s. 7386-7393Number of pages 8 s. Language eng - English Country US - United States Keywords mass ; exchange ; identification ; complex ; proteins ; sequence OECD category Microbiology R&D Projects EF18_046/0015974 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CIISB II - 90127 - Masarykova univerzita Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 001225426900001 EID SCOPUS 85192254871 DOI https://doi.org/10.1021/acs.analchem.3c04161 Annotation Covalent labeling in combination with mass spectrometry is a powerful approach used in structural biology to study protein structures, interactions, and dynamics. Recently, the toolbox of covalent labeling techniques has been expanded with fast fluoroalkylation of proteins (FFAP). FFAP is a novel radical labeling method that utilizes fluoroalkyl radicals generated from hypervalent Togni reagents for targeting aromatic residues. This report further demonstrates the benefits of FFAP as a new method for structural characterization of therapeutic antibodies and interaction interfaces of antigen-antibody complexes. The results obtained from human trastuzumab and its complex with human epidermal growth factor receptor 2 (HER2) correlate well with previously published structural data and demonstrate the potential of FFAP in structural biology. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2025 Electronic address https://pubs.acs.org/doi/10.1021/acs.analchem.3c04161
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