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Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice
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SYSNO ASEP 0586001 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice Author(s) Umek, N. (SI)
Pušnik, L. (SI)
Ugwoke, Ch. K. (SI)
Šink, Ž. (SI)
Horvat, S. (SI)
Janáček, Jiří (FGU-C) RID, ORCID
Cvetko, E. (SI)Source Title Biomolecules and Biomedicine - ISSN 2831-0896
Roč. 24, č. 3 (2024), s. 582-592Number of pages 11 s. Language eng - English Country BA - Bosnia and Herzegovina Keywords 1 diabetes mellitus (T1DM) ; skeletal muscle ; myosin heavy chain (MyHC) isoforms ; capillary network analysis ; muscle fiber composition ; three-dimensional (3D) image analysis OECD category Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology R&D Projects EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure Czech-BioImaging III - 90250 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 001180538300001 EID SCOPUS 85192428614 DOI https://doi.org/10.17305/bb.2023.9843 Annotation It is not well-understood how type 1 diabetes (T1DM) affects skeletal muscle histological phenotype, particularly capillarisation. This study aimed to analyze skeletal muscle myosin heavy chain (MyHC) fibre type changes and 3D capillary network characteristics in experimental T1DM mice. Female C57BL/6J-OlaHsd mice were categorized into streptozotocin (STZ)-induced diabetic (n = 12) and age-matched non-diabetic controls (n =12). The muscle fibre phenotype of the soleus, gluteus maximus, and gastrocnemius muscles were characterized based on the expression of MyHC isoforms, while capillaries of the gluteus maximus were assessed with immunofluorescence staining, confocal laser microscopy and 3D image analysis. STZ-induced diabetic mice exhibited elevated glucose levels, reduced body weight, and prolonged thermal latency, verifying the T1DM phenotype. In both T1DM and non-diabetic mice, the gluteus maximus and gastrocnemius muscles predominantly expressed fast-twitch type 2b fibers, with no significant differences noted. However, the soleus muscle in non-diabetic mice had a greater proportion of type 2a fibers and comparable type 1 fiber densities (26.2 ± 14.6% vs 21.9 ± 13.5%) relative to diabetic mice. T1DM mice showed reduced fiber diameters (P = 0.026), and the 3D capillary network analysis indicated a higher capillary length per muscle volume in the gluteus maximus of diabetic mice compared to controls (P < 0.05). Overall, T1DM induced significant changes in the skeletal muscle, including shifts in MyHC fibre types, decreased fibre diameters, and increased relative capillarisation, possibly due to muscle fibre atrophy. Our findings emphasize the superior detail provided by the 3D analytical method for characterizing skeletal muscle capillary architecture, highlighting caution in interpreting 2D data for capillary changes in T1DM. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2025 Electronic address https://doi.org/10.17305/bb.2023.9843
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