Outdoor cultivation and metabolomics exploration ofi Chlamydomonas/i engineered for bisabolene production

0585726 - MBÚ 2025 RIV NL eng J - Journal Article
Sawant, K. R. - Sarnaik, A. P. - Singh, Rabinder - Savvashe, P. - Baier, T. - Kruse, O. - Jutur, P. P. - Lali, A. - Pandit, R. A.
Outdoor cultivation and metabolomics exploration ofi Chlamydomonas/i engineered for bisabolene production.
Bioresource Technology. Roč. 398, April 2024 (2024), č. článku 130513. ISSN 0960-8524. E-ISSN 1873-2976
Institutional support: RVO:61388971
Keywords : phototrophic production * reinhardtii * carbon * Natural dynamic conditions * Algal bio-refinery * Growth engineering * Bicarbonate supplementation * Carotenoids
OECD category: Microbiology
Impact factor: 11.4, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0960852424002165?via%3Dihub

Demonstrating outdoor cultivation of engineered microalgae at considerable scales is essential for their prospective large-scale deployment. Hence, this study focuses on the outdoor cultivation of an engineered Chlamydomonas reinhardtii strain, 3XAgBs-SQs, for bisabolene production under natural dynamic conditions of light and temperature. Our preliminary outdoor experiments showed improved growth, but frequent culture collapses in conventional Tris-acetate-phosphate medium. In contrast, modified high-salt medium (HSM) supported prolonged cell survival, outdoor. However, their subsequent outdoor scale-up from 250 mL to 5 L in HSM was effective with 10 g/L bicarbonate supplementation. Pulse amplitude modulation fluorometry and metabolomic analysis further validated their improved photosynthesis and uncompromised metabolic fluxes towards the biomass and the products (natural carotenoids and engineered bisabolene). These strains could produce 906 mg/L bisabolene and 54 mg/L carotenoids, demonstrating the first successful outdoor photoautotrophic cultivation of engineered C. reinhardtii, establishing it as a onecell twowells biorefinery.
Permanent Link: https://hdl.handle.net/11104/0353435