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Poly‑β‑hydroxybutyrate production by Synechocystis MT_a24 in a raceway pond using urban wastewater
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SYSNO ASEP 0584992 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Poly‑β‑hydroxybutyrate production by Synechocystis MT_a24 in a raceway pond using urban wastewater Author(s) Grivalský, Tomáš (MBU-M) ORCID
Lakatos, Gergely Erno (MBU-M) ORCID
Štěrbová, Karolína (MBU-M)
Manoel, Joao Camara (MBU-M) ORCID
Beloša, Romana (MBU-M)
Divoká, Petra (MBU-M) ORCID
Kopp, J. (AT)
Kriechbaum, R. (AT)
Spadiut, O. (AT)
Zwirzitz, A. (AT)
Trenzinger, K. (AT)
Masojídek, Jiří (MBU-M) RID, ORCIDArticle number 44 Source Title Applied Microbiology and Biotechnology. - : Springer - ISSN 0175-7598
Roč. 108, č. 1 (2024)Number of pages 12 s. Language eng - English Country DE - Germany Keywords nostoc-muscorum agardh ; carbon-dioxide ; chlorophyll-a ; cyanobacterium ; growth ; accumulation ; carotenoids ; cells ; ph ; Biodegradable plastics ; Polyhydroxybutyrate ; Synechocystis ; Urban wastewater ; Raceway pond cultivation OECD category Microbiology Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 001136853300001 EID SCOPUS 85181399207 DOI https://doi.org/10.1007/s00253-023-12924-3 Annotation Poly-beta-hydroxybutyrate (PHB) is a potential source of biodegradable plastics that are environmentally friendly due to their complete degradation to water and carbon dioxide. This study aimed to investigate PHB production in the cyanobacterium Synechocystis sp. PCC6714 MT_a24 in an outdoor bioreactor using urban wastewater as a sole nutrient source. The culture was grown in a thin-layer raceway pond with a working volume of 100 L, reaching a biomass density of up to 3.5 g L-1 of cell dry weight (CDW). The maximum PHB content was found under nutrient-limiting conditions in the late stationary phase, reaching 23.7 +/- 2.2% PHB per CDW. These data are one of the highest reported for photosynthetic production of PHB by cyanobacteria, moreover using urban wastewater in pilot-scale cultivation which multiplies the potential of sustainable cultivation approaches. Contamination by grazers (Poterioochromonas malhamensis) was managed by culturing Synechocystis in a highly alkaline environment (pH about 10.5) which did not significantly affect the culture growth. Furthermore, the strain MT_a24 showed significant wastewater nutrient remediation removing about 72% of nitrogen and 67% of phosphorus. These trials demonstrate that the photosynthetic production of PHB by Synechocystis sp. PCC6714 MT_a24 in the outdoor thin-layer bioreactor using urban wastewater and ambient carbon dioxide. It shows a promising approach for the cost-effective and sustainable production of biodegradable carbon-negative plastics. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2025 Electronic address https://link.springer.com/article/10.1007/s00253-023-12924-3
Number of the records: 1