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Variations in the enzymatic activity of S1-type nucleases results from differences in their active site structures
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SYSNO ASEP 0584258 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Variations in the enzymatic activity of S1-type nucleases results from differences in their active site structures Author(s) Krela, Rafal (BC-A) RID, ORCID
Poreba, M. (PL)
Lesniewicz, K. (PL)Number of authors 3 Article number 130424 Source Title Biochimica et Biophysica Acta-General Subjects. - : Elsevier - ISSN 0304-4165
Roč. 1867, č. 10 (2023)Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords Non-zinc-dependent activity ; S1-like nuclease ; Active-site ; Acanthamoeba castellanii ; Physcomitrella patens Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 001058918800001 EID SCOPUS 85165714520 DOI 10.1016/j.bbagen.2023.130424 Annotation Background: S1-like nucleases are widespread enzymes commonly used in biotechnology and molecular biology. Although it is commonly believed that they are mainly Zn2+ dependent acidic enzymes, we have found that numerous members of this family deviate from this rule. Therefore, in this work, we decided to check how broad is the range of non-zinc-dependent S1-like nucleases and what is the molecular basis of their activities. Methods: S1-like nucleases chosen for analysis were achieved through heterologous expression in appropriate eukaryotic hosts. To characterize nucleases active-site properties, point mutations were introduced in selected positions. The enzymatic activities of wild-type and mutant nucleases were tested by in-gel nuclease activity assay. Results: We discovered that S1-like nucleases encoded by non-vascular plants and single-celled protozoa, like their higher plant homologues, exhibit a large variety of catalytic properties. We have shown that these individual properties are determined by specific non-conserved active site residues. Conclusions: Our findings demonstrate that mutations that occur during evolution can significantly alter the catalytic properties of S1-like nucleases. As a result, different ions can compete for particular S1-type nucleases active sites. This phenomenon undermines the existing classification of S1-like nucleases. General significance: Our findings have numerous implications for applications and understanding the S1-like nucleases biological functions. For example, new biotechnological applications should take into account their unexpected catalytic properties. Moreover, these results demonstrate that the trinuclear zinc-based model commonly used to characterize the catalytic activities of S1-like nucleases is insufficient to explain the actions of non-zinc-dependent members of this family. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2024 Electronic address https://www.sciencedirect.com/science/article/pii/S0304416523001228?via%3Dihub
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