Number of the records: 1
Nearly (?) sterile avian egg in a passerine bird
- 1.
SYSNO ASEP 0582395 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nearly (?) sterile avian egg in a passerine bird Author(s) Těšický, Martin (UBO-W) ORCID, RID, SAI
Schmiedová, Lucie (UBO-W) RID, ORCID, SAI
Krajzingrová, T. (CZ)
Samblas, M. G. (CZ)
Bauerová, P. (CZ)
Kreisinger, J. (CZ)
Vinkler, M. (CZ)Number of authors 7 Article number fiad164 Source Title FEMS Microbiology Ecology. - : Oxford University Press - ISSN 0168-6496
Roč. 100, č. 1 (2024)Number of pages 13 s. Language eng - English Country US - United States Keywords egg microbiome ; embryo ; gastrointestinal tract microbiota ; passerine bird ; pathogenic bacteria ; sterile egg Subject RIV EG - Zoology OECD category Zoology Method of publishing Open access Institutional support UBO-W - RVO:68081766 UT WOS 001146416500002 EID SCOPUS 85182908701 DOI 10.1093/femsec/fiad164 Annotation During early ontogeny, microbiome affects development of the gastrointestinal tract, immunity, and survival in vertebrates. Bird eggs are thought to be (1) initially sterile (sterile egg hypothesis) and (2) colonized after oviposition through horizontal trans-shell migration, or (3) initially seeded with bacteria by vertical transfer from mother oviduct. To date, however, little empirical data illuminate the contribution of these mechanisms to gut microbiota formation in avian embryos. We investigated microbiome of the egg content (day 0, E0-egg), embryonic gut at day 13 (E13) and female faeces in a free-living passerine, the great tit (Parus major), using a methodologically advanced procedure combining 16S rRNA gene sequencing and microbe-specific qPCR assays. Our metabarcoding revealed that the avian egg is (nearly) sterile, but acquires a slightly richer microbiome during the embryonic development. Of the three potentially pathogenic bacteria targeted by qPCR, only Dietzia was found in E0-egg (yet also in negative controls), E13 gut and female samples, which might indicate possible vertical transfer. Unlike in poultry, we have shown that major bacterial colonization of the gut in passerines does not occur before hatching. We emphasize that protocols that carefully check for environmental contamination are critical in studies with low-bacterial biomass samples. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2025 Electronic address https://academic.oup.com/femsec/article/100/1/fiad164/7480268?login=true
Number of the records: 1