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The Ameliorating Effects of n-3 Polyunsaturated Fatty Acids on Liver Steatosis Induced by a High-Fat Methionine Choline-Deficient Diet in Mice
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SYSNO ASEP 0581927 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Ameliorating Effects of n-3 Polyunsaturated Fatty Acids on Liver Steatosis Induced by a High-Fat Methionine Choline-Deficient Diet in Mice Author(s) Šmídl, V. (CZ)
Dvořák, K. (CZ)
Stehnová, K. (CZ)
Strnad, Hynek (UMG-J) RID
Rubert, J. (CZ)
Stříteský, J. (CZ)
Staňková, B. (CZ)
Stránská, M. (CZ)
Hajšlová, J. (CZ)
Brůha, R. (CZ)
Vítek, L. (CZ)Number of authors 0 Article number 17226 Source Title International Journal of Molecular Sciences. - : MDPI - ISSN 1661-6596
Roč. 24, č. 24 (2023)Number of pages 21 s. Language eng - English Country CH - Switzerland Keywords lipidome ; lipids ; n-3 fatty acids ; non-alcoholic fatty liver disease ; non-alcoholic steatohepatitis OECD category Biochemistry and molecular biology Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 001130968100001 EID SCOPUS 85180731947 DOI 10.3390/ijms242417226 Annotation The pathogenesis of non-alcoholic fatty liver disease (NAFLD) is associated with abnormalities of liver lipid metabolism. On the contrary, a diet enriched with n-3 polyunsaturated fatty acids (n-3-PUFAs) has been reported to ameliorate the progression of NAFLD. The aim of our study was to investigate the impact of dietary n-3-PUFA enrichment on the development of NAFLD and liver lipidome. Mice were fed for 6 weeks either a high-fat methionine choline-deficient diet (MCD) or standard chow with or without n-3-PUFAs. Liver histology, serum biochemistry, detailed plasma and liver lipidomic analyses, and genome-wide transcriptome analysis were performed. Mice fed an MCD developed histopathological changes characteristic of NAFLD, and these changes were ameliorated with n-3-PUFAs. Simultaneously, n-3-PUFAs decreased serum triacylglycerol and cholesterol concentrations as well as ALT and AST activities. N-3-PUFAs decreased serum concentrations of saturated and monounsaturated free fatty acids (FAs), while increasing serum concentrations of long-chain PUFAs. Furthermore, in the liver, the MCD significantly increased the hepatic triacylglycerol content, while the administration of n-3-PUFAs eliminated this effect. Administration of n-3-PUFAs led to significant beneficial differences in gene expression within biosynthetic pathways of cholesterol, FAs, and pro-inflammatory cytokines (IL-1 and TNF-α). To conclude, n-3-PUFA supplementation appears to represent a promising nutraceutical approach for the restoration of abnormalities in liver lipid metabolism and the prevention and treatment of NAFLD. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2024 Electronic address https://www.mdpi.com/1422-0067/24/24/17226
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